US 2009/0023161 A1 · STRUCTURAL BASIS FOR TARGETING HIV-1 GAG PROTEINS TO THE PLASMA MEMBRANE FOR VIRUS ASSEMBLY

Abstract

The present invention provides for testing methods to determine an effective testing agent that affects the activity of the HIV Gag protein at the plasma membrane of a cell, and specifically, effecting changes in the structural conformation of at least one fatty acid of PI(4,5)P2, a member of a family of differentially phosphorylated phosphatidylinositides, wherein inhibition of the extension of such fatty acid into the MA domain of the Gag protein reduces binding of Gag to the plasma membrane, thereby inhibiting virus particle assembly and subsequent replication of the HIV virus.

Claims18

Dependent claims

A testing method comprising:

(a) contacting a test compound with PI(4,5)P2 or a fragment thereof;
(b) determining the ability of the test compound to bind to the PI(4,5)P2 and exhibiting at least one of the following functions comprising (1) reduces binding of HIV Gag MA domain to the PI(4,5)P2 or a fragment thereof;
(2) inhibits extension of the myristic acid into the lipid bilayer or(3) inhibits the extension of a fatty acid component of PI(4,5)P2 from a lipid bilayer.

Dependencies are inferred from the claim text.

Facts

Application

12/175,809Filed 18 Jul 2008

Priority

20 Jul 2007

Published

22 Jan 2009

Term ends

Not estimatedThe source records don’t support an estimate.

Classification4 codes

G01N 33/92
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing:…
G01N 2333/161
Hiv-1, hiv-2: gag-pol, e.g. p55, p24/25, p17/18, p.7, p6, p66/68, p51/52, p31/34, p32, p40
G01N 2500/02
Screening for compounds of potential therapeutic value: screening involving studying the effect of compounds C on the interaction between…
G01N
Investigating or analysing materials by determining their chemical or physical properties

Structural basis for targeting hiv-1 gag proteins to the plasma membrane for virus assembly

Life of this patent

Structural basis for targeting hiv-1 gag proteins to the plasma membrane for virus assembly

Life of this patent

Abstract

Claims18