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US 2009/0023156 A1Published application
Methods and reagents for quantifying analytes
OwnerKarl O. Voss, Arunashree Bhamidipatiinventors
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Abstract
The present invention relates to methods and reagents for quantifying analytes. More specifically, the present invention related to methods and reagents for measuring one or more analytes present in a sample.
Claims19
A method of measuring one or more analytes in a sample comprising:
- adding a known quantity of a standard to said sample;
- loading said sample into a microfluidic device;
- separating said standard and said analyte by electrophoresis;
- immobilizing said standard and said analyte;
- detecting said standard and said analyte with at least one antibody;
- and
- comparing a signal representative of said standard to a signal representative of said analyte.
4 more elements
A method of measuring at least one analyte in a sample, characterized in that:
- one or more analytes and a known quantity of standard are resolved and immobilized in a fluid path, and then detection reagents are conveyed through the fluid path which bind to or interact with the analytes and standard and permit detection and comparison of signals generated from said immobilized analytes and standard.
A kit for measuring at least one analyte in a sample, comprising:
- one or more internal standards, and
- one or more antibodies that detect the one or more standards.
Dependencies are inferred from the claim text.