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OwnerKazuya Nishikawa, Takashi Yokogawa +1inventors
InventorsKazuya Nishikawa, Takashi Yokogawa, Satoshi Ohno
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This invention is intended to provide a protein synthesis system used for producing a tryptophan analogue-containing non-natural-amino-acid-containing protein that satisfies the following conditions: (i) tRNA that transfers a non-natural amino acid is not recognized by an endogenous aminoacyl tRNA synthetase (aaRS); (ii) it is recognized selectively by aaRS exclusive for a non-natural amino acid; and (iii) endogenous tRNA is not recognized by aaRS exclusive for a non-natural amino acid. In the eukaryotic organism-derived cell-free protein synthesis system, a yeast mitochondrial tryptophanyl tRNA synthetase is used in combination with mitochondrial tRNATrp (mt tRNATrp).
A method for producing a non-natural amino acid-containing protein comprising an amino acid at a given site of the protein mutated into a tryptophan analogue, comprising adding a mutant gene in which an amino acid codon at a given site of the protein-encoding gene has been mutated into an opal codon, a yeast mitochondrial tryptophanyl tRNA synthetase (mtWRS) of the F38A mutant and yeast mitochondrial tRNATrp (mt tRNATrp) or yeast mitochondrial opal suppressor tRNA to a protein synthesis system.
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A polypeptide having an amino acid sequence derived from the amino acid sequence as shown in SEQ ID NO:
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Dependencies are inferred from the claim text.