Plastic microfluidic separation and detection platforms

An electrophoresis separation chip comprising an anode portion, a cathode portion, and a center portion between the anode and cathode portions, wherein

• the cathode portion comprises at least one first via;
• the anode portion comprises at least one second via;
• and
• the center portion comprises a one or plurality of microfluidic channels and a detection window, each microfluidic channel having a separation region and a detection region;
• wherein
• each microfluidic channel is in fluid communication with at least one first via and at least one second via;
• the plurality of microfluidic channels are in substantially the same plane;
• the plurality of microfluidic channels do not intersect one another within the center portion;
• the detection window comprises a thin plastic;
• and
• the detection window overlaps the detection region of each microfluidic channel.