Soybean EF1A Promoter and Its Use in Constitutive Expression of Transgenic Genes in Plants
AssigneeE.I. du Pont de Nemours and Company
InventorZhongsen Li
Life of this patent
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Abstract
The promoter of a soybean translation elongation factor EF1 alpha and fragments thereof and their use in promoting the expression of one or more heterologous nucleic acid fragments in a tissue-independent or constitutive manner in plants are described.
Claims23
An isolated polynucleotide comprising:
- a) a nucleotide sequence comprising the sequence set forth in SEQ ID NO:1 or a full-length complement thereof;
- or
- b) a nucleotide sequence comprising a sequence having at least 90% sequence identity, based on the BLASTN method of alignment, when compared to the sequence set forth in SEQ ID NO:1;
- wherein said nucleotide sequence is a promoter.
An isolated polynucleotide comprising:
- (a) a nucleotide sequence comprising a fragment of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, or SEQ ID NO:7, or a full-length complement thereof;
- or
- (b) a nucleotide sequence comprising a sequence having at least 90% sequence identity, based on the BLASTN method of alignment, when compared to the nucleotide sequence of (a);
- wherein said nucleotide sequence is a promoter.
A method for expressing a yellow fluorescent protein ZS-YELLOW1 N1 in a host cell comprising:
- (a) transforming a host cell with a recombinant expression construct comprising at least one ZS-YELLOW1 N1 (YFP) nucleic acid fragment operably linked to a promoter wherein said promoter consists essentially of the nucleotide sequence set forth in SEQ ID NOs:1, 2, 3, 4, 5, 6, or 7;
- and
- (b) growing the transformed host cell under conditions that are suitable for expression of the recombinant DNA construct, wherein expression of the recombinant DNA construct results in production of increased levels of ZS-YELLOW1 N1 protein in the transformed host cell when compared to a corresponding nontransformed host cell.
A plant stably transformed with a recombinant expression construct comprising a soybean promoter and a heterologous nucleic acid fragment operably linked to said promoter, wherein said promoter is a capable of controlling expression of said heterologous nucleic acid fragment in a plant cell, and further wherein said promoter comprises a fragment of SEQ ID NO:1.
Dependencies are inferred from the claim text.