US 2008/0313770 A1 · Methods and compositions for modulating tocol content

Abstract

Compositions comprising a modulated tocol content in a plant or plant part are provided. In specific embodiments, the compositions and methods of the invention modulate tocol content by modulating the level of a polypeptide having a LEC1-type B domain in combination with modulating the level of at least one other polypeptide involved in tocol biosynthesis. Plants, plant parts, grain, seed and oil having the modulated tocol level are also provided. Methods to enhance oxidative stress tolerance of a plant or plant part, increase shelf-life, enhance the nutritional value, and improve tissue quality are also provided.

Claims35

Dependent claims

A method for increasing tocol content in a plant or part thereof, comprising

a) introducing into the plant or the plant part a heterologous polynucleotide encoding a polypeptide comprising a LEC1-type B-domain having an amino acid sequence set forth in SEQ ID NO:2 or a biologically active variant or a fragment of SEQ ID NO:2, wherein said biologically active variant comprises at least 80% sequence identity to SEQ ID NO:2, said polypeptide or the biologically active variant or fragment thereof have LEC1 activity;
b) introducing into the plant or the plant part a heterologous polynucleotide which modulates the level of at least one tocol biosynthesis polypeptide;
and,
thereby increasing the tocol content in said plant or said plant part.

1 more element

A plant or part thereof comprising

a) a heterologous polynucleotide encoding a polypeptide comprising a LEC1-type B-domain having the amino acid sequence set forth in SEQ ID NO:2 or a biologically active variant or a fragment thereof, wherein said biologically active variant comprises at least 60% sequence identity to SEQ ID NO:2, said polypeptide or the biologically active variant or fragment thereof having LEC1 activity operably linked to a first promoter active in the plant or plant part;
and,
b) a heterologous polynucleotide which modulated level of at least one heterologous tocol biosynthesis polypeptide operably linked to a second promoter active in the plant or plant part.

Dependencies are inferred from the claim text.

Methods and compositions for modulating tocol content

Life of this patent

Methods and compositions for modulating tocol content

Life of this patent

Abstract

Claims35