Polysaccharide Synthases
AssigneesAdelaide Research & Innovation Pty Ltd, Grains Research and Development Corporation, The University of Melboume
InventorsRachel Anita Burton, Geoffrey Bruce Fincher, Antony Bacic
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Abstract
The present invention relates generally to polysaccharide synthases. More particularly, the present invention relates to (1,3;1,4)-beta-D-glucan synthases. The present invention provides, among other things, methods for influencing the level of (1,3;1,4)-beta-D-glucan produced by a cell and nucleic acid and amino acid sequences which encode (1,3;1,4)-beta-D-glucan synthases.
Claims18
1-46. (canceled)
A method for modulating the level and/or activity of a (1,3;1,4)-β-D-glucan synthase in a cell, the method comprising modulating the expression of a (1,3;1,4)-β-D-glucan synthase encoding nucleic acid in the cell.
A cell comprising any one or more of:
- (i) a modulated level of (1,3;1,4)-β-D-glucan relative to a wild type cell of the same taxon;
- (ii) a modulated level and/or activity of (1,3;1,4)-β-D-glucan synthase relative to a wild type cell of the same taxon;
- and/or
- (iii) modulated expression of a (1,3;1,4)-β-D-glucan synthase encoding nucleic acid relative to a wild type cell of the same taxon.
An isolated nucleic acid molecule selected from the list consisting of:
- (i) an isolated nucleic acid molecule that encodes a (1,3;1,4)-β-D-glucan synthase;
- (ii) an isolated nucleic acid molecule comprising the nucleotide sequence set forth in any of SEQ ID NO:
- 1, SEQ ID NO:
- 3, SEQ ID NO:
- 5, SEQ ID NO:
- 7, SEQ ID NO:
- 9 and SEQ ID NO:
- 11;
- (iii) an isolated nucleic acid molecule comprising a nucleotide sequence which is at least 50% identical to the nucleotide sequence set forth in any of SEQ ID NO:
- 1, SEQ ID NO:
- 3, SEQ ID NO:
- 5, SEQ ID NO:
- 7, SEQ ID NO:
- 9 and SEQ ID NO:
- 11;
- (iv) an isolated nucleic acid molecule comprising a nucleotide sequence which encodes the amino acid sequence set forth in any of SEQ ID NO:
- 2, SEQ ID NO:
- 4, SEQ ID NO:
- 6, SEQ ID NO:
- 8, SEQ ID NO:
- 10 and SEQ ID NO:
- 12;
- (v) an isolated nucleic acid molecule comprising a nucleotide sequence which hybridises to any of (i) to (iv) under stringent conditions;
- (vi) an isolated nucleic acid molecule comprising a nucleotide sequence which is the complement or reverse complement of the nucleotide sequence of the isolated nucleic acid molecule of any of (i) to (v);
- and/or
- (vii) a fragment of any one of the isolated nucleic acid molecule of any of (i) to (vi).
An isolated polypeptide selected from the list consisting of:
- (i) an isolated polypeptide comprising an amino acid sequence encoding a (1,3;1,4)-β-D-glucan synthase;
- (ii) an isolated polypeptide comprising the amino acid sequence set forth in any of SEQ ID NO:
- 2, SEQ ID NO:
- 4, SEQ ID NO:
- 6, SEQ ID NO:
- 8, SEQ ID NO:
- 10 and SEQ ID NO:
- 12;
- (iii) an isolated polypeptide comprising an amino acid sequence comprising at least 50% identity to the amino acid sequence set forth in any of SEQ ID NO:
- 2, SEQ ID NO:
- 4, SEQ ID NO:
- 6, SEQ ID NO:
- 8, SEQ ID NO:
- 10 and SEQ ID NO:
- 12;
- (iv) an isolated polypeptide comprising an amino acid sequence encoded by the nucleotide sequence set forth in any of SEQ ID NO:
- 1, SEQ ID NO:
- 3, SEQ ID NO:
- 5, SEQ ID NO:
- 7, SEQ ID NO:
- 9 and SEQ ID NO:
- 11;
- and/or
- (v) a fragment of the isolated polypeptide of any one of (i) to (iv).
Dependencies are inferred from the claim text.