Agents, compositions and methods for treating pathologies in which regulating an ache-associated biological pathway is beneficial

A method of regulating an expression level ratio of AChE-S and AChE-R and/or AChE-S mRNA and AChE-R mRNA splice variants in AChE expressing cells comprising subjecting the AChE gene expressing cells to an agent capable of regulating a function of a miRNA component associated with regulating the expression level ratio of AChE-S and AChE-R splice variants, thereby regulating the expression level of the AChE-S and AChE-R splice variants in the AChE expressing cells.

A method of treating a pathology related to an AChE-associated biological pathway, the method comprising administering to a subject in need thereof an agent capable of regulating a function of a miRNA component of the AChE-associated biological pathway, thereby treating the pathology.

A method of altering differentiation and/or proliferation of hematopoietic progenitor and/or stem cells, the method comprising subjecting the progenitor and/or stem cells to an agent capable of regulating a function a miRNA component of an AChE-associated biological pathway in the progenitor and/or stem cells, thereby altering differentiation and/or proliferation of the hematopoietic progenitor and/or stem cells.

A method of regulating apoptosis in cells and/or a tissue of a subject in need thereof, the method comprising subjecting the cells and/or the tissue of the subject to an agent capable of regulating a function a miRNA component of an AChE-associated biological pathway in the cells and/or tissue, thereby regulating apoptosis in the cells and/or the tissue of the subject.

A method of diagnosing a pathology associated with abnormal function of a miRNA component of an AChE-associated biological pathway in a subject, the method comprising obtaining a biological sample from the subject and determining a level of the miRNA in cells of said biological sample, wherein a level of the miRNA above or below a predetermined threshold or range is indicative of a presence of a pathology associated with abnormal function of the miRNA.

An isolated polynucleotide comprising a nucleic acid sequence which comprises at least 10 consecutive nucleotides of the nucleotide sequence set forth in SEQ ID NO:1, with the proviso that isolated polynucleotide is not identical to the sequence set forth in SEQ ID NO:1.

An isolated polynucleotide comprising a nucleic acid sequence of 10-50 bases and capable of hybridizing in cells under physiological conditions with an RNA molecule which comprises a nucleotide sequence as set forth in SEQ ID NO:2, with the proviso that isolated polynucleotide is not identical to the sequence set forth in SEQ ID NO:1.

An isolated polynucleotide comprising a nucleic acid sequence which comprises at least 10 consecutive nucleotides from the nucleotide sequence set forth by SEQ ID NO:2.

An isolated polynucleotide comprising a nucleic acid sequence of 10-50 bases and capable of hybridizing in cells under physiological conditions with an RNA molecule which comprises a nucleotide sequence as set forth in SEQ ID NO:1.

An isolated polynucleotide comprising a nucleic acid sequence which comprises at least 20 consecutive nucleotides from the nucleotide sequence set forth in SEQ ID NO:13, with the proviso that isolated polynucleotide is not identical to the sequence set forth in SEQ ID NO:13.