Single-Step Platform for On-Chip Integration of Bio-Molecules

A process of attaching a screenable moiety to at least one pre-selected area of a solid substrate, the process comprising:

• providing a solid substrate having at least one functional group on a surface thereof;
• providing a compound having a functionalized group capable of binding to said solid substrate, a photoactivatable group capable of generating a reactive group upon exposure to a light source, said reactive group being for binding said screenable moiety, and a polymer capable of forming a monolayer on said solid substrate and having said functionalized group and said photoactivatable group attached thereto;
• contacting said compound with said solid substrate, to thereby provide a solid substrate having a monolayer of said polymer attached thereto, wherein said polymer has said photoactivatable group attached thereto;
• exposing said at least one pre-selected area of said solid substrate having said monolayer of said polymer attached thereto to a light, to thereby generate said reactive group at said pre-selected area;
• and
• binding said screenable moiety to said reactive group, thereby attaching the screenable moiety to the pre-selected area of the solid support.

A process of preparing an array of screenable moieties, the process comprising:

• providing a solid substrate having a plurality of functional groups on a surface thereof;
• providing a compound having a functionalized group capable of binding to said solid substrate, a photoactivatable group capable of generating a reactive group upon exposure to a light source, said reactive group being for binding a screenable moiety;
• and a polymer capable of forming a monolayer on said solid substrate and having said functionalized group and said photoactivatable group attached thereto;
• contacting said compound to said solid substrate, to thereby provide a solid substrate having a plurality of monolayers of said polymer attached thereto, wherein said polymer has said photoactivatable groups attached thereto;
• exposing a first pre-selected area of said solid substrate having said monolayer of said polymer attached thereto to a light, to thereby generate a reactive group at said first pre-selected area;
• and
• binding a screenable moiety to said reactive group at said first pre-selected area;
• exposing a second pre-selected area of said solid substrate having said monolayer of said polymer attached thereto to a light, to thereby generate a reactive group at said second pre-selected area;
• binding a second screenable moiety to said reactive group at said second pre-selected area;
• consecutively exposing an Nth pre-selected area of said solid substrate having said monolayer of said polymer attached thereto to a light, to thereby generate a reactive group at said Nth pre-selected area;
• and
• binding an Nth screenable moiety to said reactive group at said Nth pre-selected area, thereby providing an array of screenable moieties.