Sequences for Differential Diagnostic of Ehrlichia Ruminantium and Use Thereof
AssigneesCentre de Cooperation Internationale en Recherche Agronomique pour le Developpement (CIRAD), Centre National De La Recherche Scientifique
InventorsRoger Frutos, Conception Ferraz, Jacques Demaille, Dominique Martinez
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Abstract
The invention provides genes that are unique either to Ehrlichia ruminantium strain Gardel or to Ehrlichia ruminantium strain Welgevonden, or allelic couples which are present in both strains but whose sequences differ between the two strains, as genetic markers to differentiate between these two strains. The invention also provides diagnostic methods using these genetic markers.
Claims6
1) A method for discriminating between Ehrlichia ruminantium strain Gardel and Ehrlichia ruminantium strain Welgevonden, wherein said method comprises the detection of the presence or the absence, in the bacteria to be tested, of at least one orphan gene selected among:
- ERGA_CDS—04340 (SEQ ID NO:
- 1)
- ERGA_CDS—04980 (SEQ ID NO:
- 2)
- ERGA_CDS—05590 (SEQ ID NO:
- 3)
- ERGA_CDS—05600 (SEQ ID NO:
- 4)
- ERGA_CDS—07580 (SEQ ID NO:
- 5)
- ERWE_CDS—08330 (SEQ ID NO:
- 6)
3) An isolated polynucleotide selected among:
- a) ERGA_CDS—05590, ERGA_CDS—07580, ERGA_CDS—04980, ERGA_CDS—05600, ERGA_CDS—08330, ERGA_CDS—04340, or their complement;
- b) a fragment of at least 15 consecutive base pairs of a polynucleotide a);
- c) a polynucleotide of at least 15 bp that hybridizes selectively, under stringent hybridization conditions, with a polynucleotide a).
4) A DNA array comprising at least one polynucleotide selected among:
- a) ERGA_CDS—05590, ERGA_CDS—07580, ERGA_CDS—04980, ERGA_CDS—05600, ERGA_CDS—08330, and ERGA_CDS—04340, or their complement;
- b) a fragment of at least 30 consecutive base pairs of a polynucleotide a);
- c) a polynucleotide of at least 30 bp that hybridizes selectively, under stringent hybridization conditions, with a polynucleotide a).
Dependencies are inferred from the claim text.