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US 2008/0305044 A1Published application
Engineered Antibodies and Immunoconjugates
AssigneeSeattle Genetics, Inc.
InventorsCharlotte McDonagh, Paul Carter
Life of this patent
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Abstract
Antibody drug conjugates with predetermined sites and stoichiometries of drug attachment are provided. Also provided are methods of using antibody drug conjugates.
Claims40
An immunoconjugate comprising:
- an engineered antibody having (a) a functionally active antigen-binding region for a target antigen, (b) at least one interchain cysteine residue, (c) at least one amino acid substitution of an interchain cysteine residue, and (d) a diagnostic, preventative or therapeutic agent conjugated to at least one interchain cysteine residue.
A method for preparing an immunoconjugate, comprising:
- (a) culturing a host cell expressing an engineered antibody, the engineered antibody comprising (i) a functionally active antigen-binding region for a target antigen, (ii) at least one interchain cysteine residue, and (iii) at least one amino acid substitution of an interchain cysteine residue, the host cells being transformed or transfected with an isolated nucleic acid encoding the engineered antibody;
- (b) recovering the antibody from the cultured host cells or the culture medium;
- and
- (c) conjugating a diagnostic, preventative or therapeutic agent to the at least one interchain cysteine residue.
1 more element
Dependencies are inferred from the claim text.