US 2008/0287318 A1 · Primers and Probes for Detecting Genital Hpv Genotypes

Abstract

The invention relates to oligonucleotides, which are suited as primers for amplifying DNA of genital human papilloma viruses (HPV), to oligonucleotides, which are suited for use as probes for typifying genital HPV genotypes, to methods for amplifying the DNA of genital human papilloma viruses (HPV), to methods for detecting and/or identifying genital HPV genotypes, to nucleotide microarrays containing the oligonucleotides, to kits and to the use of the oligonucleotides for amplifying or typifying genital HPV genotypes, for the diagnosis and/or early diagnosis of diseases and for producing agents for diagnosing diseases.

Claims69

Dependent claims

An oligonucleotide that may be used as a primer, in particular a forward primer, to amplify a nucleic acid region of a genital human papilloma virus (HPV) and that has the sequence 5′-CAR GCI AAA WWW KTD MR GAY TGT G-3′, or 5′-CAR GCN AAA WWW KTD MR GAY TGT G-3′ (SEQ ID no. 1), wherein R=A, or G, W=T, or A, K=T, or G, I=inosine, N=A, T, G, or C, D=A, T, or G, and Y=C, or T.

An oligonucleotide that may be used as a probe for detecting and/or for identifying genital HPV genotypes selected from the group comprising:

1) an oligonucleotide having the nucleotide sequence recited in SEQ ID no. 117 for detecting and/or identifying the HPV6 genotype,
2) an oligonucleotide having the nucleotide sequence recited in SEQ ID no. 118 for detecting and/or identifying the HPV11 genotype,
3) an oligonucleotide having the nucleotide sequence recited in SEQ ID no. 19 for detecting and/or identifying the HPV16 genotype,
4) an oligonucleotide having the nucleotide sequence recited in SEQ ID no. 119 for detecting and/or identifying the HPV18 genotype,
5) an oligonucleotide having the nucleotide sequence recited in SEQ ID no. 120 for detecting and/or identifying the HPV31 genotype,
6) an oligonucleotide having the nucleotide sequence recited in SEQ ID no. 32 for detecting and/or identifying the HPV33 genotype,
7) an oligonucleotide having the nucleotide sequence recited in SEQ ID no. 41 for detecting and/or identifying the HPV35h genotype,
8) an oligonucleotide having the nucleotide sequence recited in SEQ ID no. 44 for detecting and/or identifying the HPV39 genotype,
9) an oligonucleotide having the nucleotide sequence recited in SEQ ID no. 48 for detecting and/or identifying the HPV40 genotype,
10) an oligonucleotide having the nucleotide sequence recited in SEQ ID no. 121 for detecting and/or identifying the HPV42 genotype,
11) an oligonucleotide having the nucleotide sequence recited in SEQ ID no. 122 for detecting and/or identifying the HPV43 genotype,
12) an oligonucleotide having the nucleotide sequence recited in SEQ ID no. 123 for detecting and/or identifying the HPV44 genotype,
13) an oligonucleotide having the nucleotide sequence recited in SEQ ID no. 124 for detecting and/or identifying the HPV45 genotype,
14) an oligonucleotide having the nucleotide sequence recited in SEQ ID no. 125 for detecting and/or identifying the HPV51 genotype,
15) an oligonucleotide having the nucleotide sequence recited in SEQ ID no. 126 for detecting and/or identifying the HPV52 genotype,
16) an oligonucleotide having the nucleotide sequence recited in SEQ ID no. 127 for detecting and/or identifying the HPV53 genotype,
17) an oligonucleotide having the nucleotide sequence recited in SEQ ID no. 128 for detecting and/or identifying the HPV56 genotype,
18) an oligonucleotide having the nucleotide sequence recited in SEQ ID no. 82 for detecting and/or identifying the HPV58 genotype,
19) an oligonucleotide having the nucleotide sequence recited in SEQ ID no. 129 for detecting and/or identifying the HPV59 genotype,
20) an oligonucleotide having the nucleotide sequence recited in SEQ ID no. 130 for detecting and/or identifying the HPV66 genotype,
21) an oligonucleotide having the nucleotide sequence recited in SEQ ID no. 131 or 132 for detecting and/or identifying the HPV68 genotype,
22) an oligonucleotide having the nucleotide sequence recited in SEQ ID no. 133 for detecting and/or identifying the HPV70 genotype,
23) an oligonucleotide having the nucleotide sequence recited in SEQ ID no. 134 for detecting and/or identifying the HPV73 genotype, and
24) an oligonucleotide having the nucleotide sequence recited in SEQ ID no. 135 for detecting and/or identifying the HPV82 genotype.

21 more elements

Dependencies are inferred from the claim text.

Primers and Probes for Detecting Genital Hpv Genotypes

Life of this patent

Primers and Probes for Detecting Genital Hpv Genotypes

Life of this patent

Abstract

Claims69