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US 2008/0287306 A1Published application
Methods and devices for sequencing nucleic acids
AssigneeHelicos BioSciences Corporation
InventorStanley N. Lapidus
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Abstract
The invention provides methods and devices for high throughput single molecule sequencing of a plurality of target nucleic acids using a universal primer. Devices of the invention comprise a plurality of oligonucleotides, each having the same sequence, bound to a solid support, and ligated to a plurality of target nucleic acids.
Claims23
A substrate for use in sequencing nucleic acids, the substrate comprising:
- a solid support;
- and
- a plurality of oligonucleotides, each having the same sequence, attached to said solid support in a spatial arrangement such that each of said oligonucleotides is individually optically resolvable,
- wherein each of said oligonucleotides comprises
- at least five nucleotides;
- a primer attachment site;
- and
- a terminal attachment site for attaching a target polynucleotide.
5 more elements
A method for sequencing nucleic acids, the method comprising:
- attaching a plurality of oligonucleotides, each having the same sequence, to a surface of a solid support in a spatial arrangement such that each of said oligonucleotides is individually optically resolvable,
- attaching each of a plurality of target polynucleotides to a different one of said oligonucleotides, producing a plurality of chimeric polynucleotides;
- exposing said chimeric polynucleotides to a primer capable of hybridizing to said oligonucleotides;
- extending said primer in the presence of one of more nucleotides comprising a detectable label;
- and
- detecting label incorporated into said extended primer, thereby to determine the sequences of said target nucleic acids.
3 more elements
Dependencies are inferred from the claim text.