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US 2008/0286773 A1Published application
Method for Typing an Individual Using Short Tandem Repeat (Str) Loci of the Genomic Dna
OwnerKlaus Benderinventor
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Abstract
The present invention relates to a novel STR typing strategy, which allows the simultaneous amplification and subsequent analysis of several (e.g. eleven) polymorphic systems with amplicon sizes of less than 270 bp. Thereby, after a PCR amplification the multiplex reaction is divided into two sets of STR multiplexes and analyzed separately. This multiplex system was particularly developed and tested for use in forensic investigations, where only limited amounts of DNA or only highly degraded DNA is available, for example, when the DNA is isolated from the roots of telogen hair.
Claims20
A method for typing an individual, comprising the steps of
- a) providing genomic DNA of the individual,
- b) amplification of at least two short tandem repeat (STR) loci of the genomic DNA using, if applicable labelled, pairs of amplification primers, wherein at least one primer is provided with a binding group,
- c) separating the amplified STR fragments by means of the binding group in at least two fractions of amplificates, and
- d) separate detection of the STR fragments of the fractions.
1 more element
Dependencies are inferred from the claim text.