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US 2008/0269072 A1Published application
Rational Probe Optimization for Detection of MicroRNAs
OwnerRonald P. Hart, Loyal A. Goffinventors
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Abstract
A method for the rational optimization of probes for the detection of miRNAs from different species is provided.
Claims19
A computer assisted method for optimizing design of probes which selectively hybridize to target miRNAs obtained from a database using a programmed computer, including a processor, an input device and an output device comprising:
- a) inputting into the programmed computer miRNA sequence data,
- b) inputting upper and lower ranges of sequence length;
- c) inputting upper and lower ranges of Tm;
- d) determining using the processor those probes which satisfy the inputted Tm parameters and sequence length following truncation of the sequences at either the 3′ or 5′ end of said sequence;
- and
- e) outputting those probes that satisfy the inputted Tm parameters.
3 more elements
A method for rational probe optimization for detection of Mi RNA molecules comprising:
- a) providing a database of known miRNA sequences;
- b) performing the miRMAX algorithm on said sequences to identify probes having enhanced sequence specificity, substantially similar hybridization temperatures and sequence length;
- and
- c) obtaining the probe sequences identified in step b) and optionally synthesizing the same.
1 more element
Dependencies are inferred from the claim text.