Compositions and Methods of Use of Response Regulators
AssigneePioneer Hi-Bred International, Inc.
InventorsNorbert Brugiere, Robert J. Meister, Shoba Sivasnakar
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Abstract
Methods and compositions for modulating plant development are provided. Methods employing type A Response Regulators (RR) are provided. The type A RR sequences are used in a variety of methods including modulating root development, modulating leaf and/or shoot development, modulating shoot regeneration from callus, modulating tolerance under abiotic stress, modulating yield, modulating cytokinin level/activity, and modulating plant responsiveness to cytokinin. Transformed plants, plant cell, tissues, seed, and expression vectors are also provided.
Claims14
A method for affecting growth of a plant, comprising introducing into said plant an expression construct directing modulation of expression of a polynucleotide, said polynucleotide comprising a nucleotide sequence selected from the group consisting of:
- (a) a nucleotide sequence comprising SEQ ID NO:
- 1, 4 or 11;
- (b) a nucleotide sequence encoding an amino acid sequence comprising SEQ ID NO:
- 2, 5 or 9;
- (c) a nucleotide sequence having at least 85% sequence identity to SEQ ID NO:
- 1, 4 or 11, wherein said polynucleotide encodes a polypeptide having response regulator activity;
- and
- (d) a nucleotide sequence that hybridizes under stringent conditions to the complement of the polynucleotide of a), wherein said stringent conditions comprise hybridization in 50% formamide, 1 M NaCl, 1% SDS at 37° C., and a wash in 0.1×SSC at 60° C. to 65° C.;
- wherein said plant exhibits an altered phenotype.
A method for enhancing the amount or rate of in vitro callus growth comprising transforming said callus, or a progenitor cell, with an expression construct directing modulation of expression of a polynucleotide, said polynucleotide comprising a nucleotide sequence selected from the group consisting of:
- (a) a nucleotide sequence comprising SEQ ID NO:
- 1, 4 or 11;
- (b) a nucleotide sequence encoding an amino acid sequence comprising SEQ ID NO:
- 2, 5 or 9;
- (c) a nucleotide sequence having at least 85% sequence identity to SEQ ID NO:
- 1, 4 or 11, wherein said polynucleotide encodes a polypeptide having response regulator activity;
- and
- (d) a nucleotide sequence that hybridizes under stringent conditions to the complement of the polynucleotide of a), wherein said stringent conditions comprise hybridization in 50% formamide, 1 M NaCl, 1% SDS at 37° C., and a wash in 0.1×SSC at 60° C. to 65° C.;
- wherein the amount or rate of callus growth is increased.
Dependencies are inferred from the claim text.