Transcytosis of Adeno-Associated Viruses
OwnerJohn A. Chiorini, Giovanni Di Pasqualeinventors
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Abstract
The present invention provides methods of transcytosing barrier epithelial cells using adeno-associated virus-4 (AAV4), adeno-associated virus-5 (AAV5), adeno-associated virus-7 (AAV7), bovine adeno-associated virus (BAAV), and vectors and particles derived therefrom. In addition, the present invention provides methods of delivering a nucleic acid across the barrier epithelia using the AAV4, AAV5, AAV7, and BAAV vectors and particles.
Claims25
A method of delivering a heterologous nucleic acid across an epithelial barrier comprising delivering to the epithelial barrier an AAV vector comprising the heterologous nucleic acid.
A method of transcytosing epithelial cells of a human subject comprising administering to the subject an AAV vector comprising a heterologous nucleic acid.
A method of delivering a heterologous nucleic acid across human airway epithelial cells, comprising delivering to the cells a BAAV vector comprising the nucleic acid.
A method of delivering a heterologous nucleic acid across human cerebral microvascular endothelial cells, comprising delivering to the cells a BAAV vector comprising the nucleic acid.
8-9. (canceled)
A method of delivering a heterologous nucleic acid across human enterocytes, comprising delivering to the cells a AAV5 vector comprising the nucleic acid.
11-16. (canceled)
A method of delivering a heterologous nucleic acid across an epithelial barrier of the lung, comprising delivering to the lung a BAAV vector comprising the nucleic acid.
A method of delivering a heterologous nucleic acid across an epithelial barrier in the brain, comprising delivering to the brain a BAAV vector comprising the nucleic acid.
21-26. (canceled)
A method of transcytosing lung epithelial cells of a subject comprising contacting the lung epithelial cells of the subject with a BAAV vector comprising a heterologous nucleic acid.
A method of transcytosing CNS epithelial cells of a subject comprising contacting the CNS epithelial cells of the subject with a BAAV vector comprising a heterologous nucleic acid.
31-36. (canceled)
A method of delivering a heterologous nucleic acid across an epithelial barrier in the gut, comprising delivering to the gut an AAV5 vector comprising the nucleic acid.
A method of transcytosing gut epithelial cells of a subject comprising contacting the gut epithelial cells of the subject with an AAV5 vector comprising a heterologous nucleic acid.
41-68. (canceled)
Dependencies are inferred from the claim text.