Assay for diabetes
AssigneeMinomic Pty Ltd
InventorsBradley John Walsh, Geoffrey Bruce Randall, Steven Brian Wilson
Life of this patent
Estimating the term…
Abstract
An assay for testing a subject for diabetes or a predisposition to diabetes including analyzing a biological fluid from a subject for the presence of one or more proteins selected from the group consisting of Alpha 2 macroglobulin, Apolipoprotein AII, Immunoglobulin alpha heavy chain constant region, Immunoglobulin mu chain C region, Chain A of Human IgA1, Inter-alpha-trypsin inhibitor heavy chain H4 precursor, and Apolipoprotein B11; wherein detection of the protein is indicative of diabetes or a predisposition to diabetes in the subject.
Claims15
An assay for testing a subject for diabetes or a predisposition to diabetes comprising:
- analyzing a biological fluid from a subject for the presence of one or more proteins selected from the group consisting of Alpha 2 macroglobulin, Apolipoprotein A1, Immunoglobulin alpha heavy chain constant region, Immunoglobulin mu chain C region, Chain A of Human IgA1, Inter-alpha-trypsin inhibitor heavy chain H4 precursor, and Apolipoprotein B-100;
- wherein detection of the protein is indicative of diabetes or a predisposition to diabetes in the subject.
An isolated peptide marker detectable in a biological sample of a subject and being indicative of diabetes or a predisposition to diabetes in a subject comprising one or more of the following amino acid sequences:
- AYIFIDEAHITQALIWLSQR,
- (SEQ ID NO:
- 1)
- LLLYAVLPTGDVIGDSAK,
- (SEQ ID NO:
- 2)
- LLLQQVSLPELPGEYSMK,
- (SEQ ID NO:
- 3)
- QGLLPVLESFK,
- (SEQ ID NO:
- 4)
- LLDNWDSVTSTFSK,
- (SEQ ID NO:
- 5)
- KEPSQGTTTFAVTSILR,
- (SEQ ID NO:
- 6)
- VFAIPPSFASIFLTK,
- (SEQ ID NO:
- 7)
- QEPSQGTTTFAVTSILR,
- (SEQ ID NO:
- 8)
- WLQGSQELPR,
- (SEQ ID NO:
- 9)
- LWAYLTIQQLLEQTVSASDADQQALR,
- (SEQ ID NO:
- 10)
- AEAQAQYSAAVAK,
- (SEQ ID NO:
- 11)
- YSQPEDSLIPFFEITVPESQLTVSQFTLPK,
- (SEQ ID NO:
- 12)
- or
- IAIANIIDEIIEK.
- (SEQ ID NO:
- 13)
An assay for testing a subject for diabetes or a predisposition to diabetes comprising:
- obtaining a urine sample from a subject;
- concentrating the urine sample;
- digesting proteins present in the concentrated urine sample to form peptides;
- optionally, separating the peptides;
- and
- analyzing the peptides for the presence of one or marker peptides having an amino acid sequence of any one of SEQ ID NOS:1 to 13, wherein the presence of marker peptides having an amino acid sequence of any one of SEQ ID NOS:1 to 13 is indicative of diabetes or a predisposition to diabetes in the subject.
Dependencies are inferred from the claim text.