Sulfonamide-based oligomers and polymers for destabilization of biological membranes
AssigneeUniversity of Utah Research Foundation
InventorsHan Chang Kang, You Han Bae
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Abstract
Oligomeric sulfonamides for use as endosomolytic reagents for transfection with polymeric or lipid-based vectors are described. A mixture of an oligomeric sulfonamide with a polymeric or lipid-based gene carrier and a nucleic acid results in a polyplex that exhibits 6-12-fold better gene expression than controls. A method of transfecting cells in vitro is carried out by contacting cultured mammalian cells with an effective amount of a polyplex.
Claims20
An oligomeric sulfonamide represented by the formula:
- wherein X is (CH3)3—C— or H2N—CH2—CH2-S—;
- R is a sulfonamide or N-substituted sulfonamide having a pKa of about 3 to 10;
- and n is about 2 to 300.
A polyplex comprising a mixture of a nucleic acid, a polymeric or lipid-based gene carrier, and an oligomeric sulfonamide represented by the formula:
- wherein X is (CH3)3—C— or H2N—CH2—CH2—S—;
- R is a sulfonamide or N-substituted sulfonamide having a pKa of about 3 to 10;
- and n is about 2 to 300.
A method for transfecting mammalian cells in vitro, the method comprising:
- (a) culturing the mammalian cells in a selected growth medium;
- and
- (b) contacting the cultured mammalian cells with an effective amount of a polyplex comprising a mixture of a nucleic acid, a positively charged polymer or lipid-based gene carrier, and an oligomeric sulfonamide represented by the formula:
- wherein X is (CH3)3—C— or H2N—CH2—CH2—S—;
- R is a sulfonamide or N-substituted sulfonamide having a pKa of about 3 to 10;
- and n is about 2 to 300.
Dependencies are inferred from the claim text.