Myo-inositol oxygenases
AssigneeCargill, Incorporated
InventorsWilliam A. Schroeder, Paula M. Hicks, Sara C. Mcfarlan, Timothy W. Abraham
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Abstract
The invention provides methods and materials related to the production of organic products such as glucuronic acid, ascorbic acid, and glucaric acid. Specifically, the invention provides cells, methods for culturing cells, isolated nucleic acid molecules, and methods and materials for producing various organic products such as glucuronic acid, ascorbic acid, and glucaric acid.
Claims46
An isolated cell comprising myo-inositol and one or more exogenous nucleic acid molecules, wherein said one or more exognous nucleic acid molecules encodes at least one polypeptide having myo-inositol oxygenase activity, and wherein said cell expresses said at least one polypeptide.
A cell comprising a genetic modification that reduces myo-inositol oxygenase activity.
A cell comprising a genetic modification that reduces L-gulonate 3-dehydrogenase activity.
An isolated nucleic acid molecule comprising a nucleic acid sequence at least about 50 percent identical to the sequence set forth in SEQ ID NO:
- 1 and wherein the polypeptide comprises myo-inositol oxygenase activity.
An isolated nucleic acid molecule that encodes a polypeptide having an amino acid sequence at least about 50 percent identical to the sequence set forth in SEQ ID NO:
- 19 and wherein the polypeptide comprises myo-inositol oxygenase activity.
Dependencies are inferred from the claim text.