Thermo-stable bio-matrix
AssigneeAgResearch Limited
InventorsVon Walter Johnson, Joan Frances Pearson
Life of this patent
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Abstract
A method for producing a thermo-stable biodegradable medium for storage of biological materials is disclosed. The method includes the preparation of a bio polymer as a matrix for biological materials. The biological material can be selected from a wide range which includes cellular organisms and micro-organisms. The medium can be used for medium to long term storage of material at room temperature with a half life of 50% for up to 6 months. The medium is water soluble and can be used as a fertilizer, spray or an inoculant.
Claims32
A method of stabilizing a bacteria composition, said method comprising the steps of:
- (a) selecting at least one powdered bio-polymer from the group consisting of:
- xanthan gum, acacia gum, guar gum, gellan, locust bean gum, and a combination thereof;
- (b) selecting a bacteria;
- (c) preparing a concentrate of the bacteria of between 10% and 100% (by weight), said concentrate being in an aqueous state;
- (d) combining the biopolymer of step (a) with the concentrate of step (c) at a ratio of approximately 1:10 to 10:1 to form a second mix;
- (e) incubating said second mix at a temperature of 5° C. to 30° C.;
- (f) adding a bio-degradable non-toxic oil to the mixture of step (e) to form a second mixture, the concentration of oil being in the range 0.1 to 10% by weight of the second mixture;
- and
- (g) agitating the second mixture at a temperature of between 5° C. and 40° C. to form a homogeneous suspension wherein a gel matrix is formed.
A method of stabilizing a bacteria composition, said method comprising the steps of:
- (a) selecting at least one powdered gum selected bio-polymer from the group consisting of:
- xanthan gum, acacia gum, guar gum, gellan, locust bean gum, and a combination thereof;
- (b) selecting a bacteria;
- (c) preparing a concentrate of the bacteria of between 10% and 100% (by weight), said concentrate being in an aqueous state;
- (d) combining the biopolymer of step (a) with the concentrate of step (c) at a temperature of between 5° C. and 40° C. and at a ratio of approximately 1:1 to form a gel matrix.
A method of stabilizing a bacteria composition, said method comprising the steps of:
- (a) selecting at least one powdered bio-polymer from the group consisting of:
- xanthan gum, acacia gum, guar gum, gellan, locust bean gum, and a combination thereof;
- (b) selecting a bacteria;
- (c) preparing a concentrate of the bacteria of between 10% and 100% (by weight), said concentrate being in an aqueous state;
- (d) combining the biopolymer of step (a) with the concentrate of step (c) at a ratio of 1:10 to 10:1 to form a second mix;
- (e) agitating the second mixture at a temperature of between 5° C. and 40° C. to form a homogeneous suspension wherein a gel matrix is formed.
A method of stabilizing a bacteria composition, said method comprising the steps of:
- (a) selecting at least one powdered bio-polymer from the group consisting of:
- xanthan gum, acacia gum, guar gum, gellan, locust bean gum, and a combination thereof;
- (b) selecting a bacteria;
- (c) preparing a concentrate of the bacteria of between 10% and 100% (by weight), said concentrate being in an aqueous state;
- (d) combining the biopolymer of step (a) with the concentrate of step (c) at a ratio of approximately 1:1 to form a second mix;
- (e) incubating said second mix at a temperature of 5° C. to 30° C.;
- (f) adding a bio-degradable non-toxic oil to the mixture of step (e) to form a second mixture, the concentration of oil being in the range 0.1 to 10% by weight of the second mixture;
- and
- (g) agitating the second mixture at a temperature of between 5° C. and 40° C. to form a homogeneous suspension wherein a gel matrix is formed.
Dependencies are inferred from the claim text.