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US 2008/0187970 A1Published application
Beta-Fructofuranosidase Variants
AssigneeMeiji Seika Kaisha, Ltd.
InventorsHirofumi Nakamura, Akitaka Nakane, Hidetoshi Kubota
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Abstract
An object of the present invention is to provide a β-fructofuranosidase variant whose reaction specificity is improved to be suitable for the production of fructooligosaccharides. According to the present invention, there is provided a β-fructofuranosidase variant consisting of a mutated amino acid sequence of SEQ ID NO: 2 or a mutated homologue thereof, which has mutations at specific amino acid residues.
Claims16
A β-fructofuranosidase variant consisting of the following amino acid sequence:
- (a) a mutated amino acid sequence of SEQ ID NO:
- 2, which has at least one mutation in amino acid residues at positions 62, 122, 128, 165, 221, 395, and 550, or
- (b) a mutated homologue of the amino acid sequence of SEQ ID NO:
- 2, which has at least one mutation in amino acid residues corresponding to the amino acid residues at positions 62, 122, 128, 165, 221, 395, and 550 of SEQ ID NO:
- 2.
2 more elements
A β-fructofuranosidase variant consisting of the following amino acid sequence:
- (c) a mutated amino acid sequence of SEQ ID NO:
- 2, which has at least one mutation in amino acid residues at positions 40, 379, and 381, or
- (d) a mutated homologue of the amino acid sequence of SEQ ID NO:
- 2, which has at least one mutation in amino acid residues corresponding to the amino acid residues at positions 40, 379, and 381 of SEQ ID NO:
- 2.
2 more elements
Dependencies are inferred from the claim text.