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AssigneeRosetta Inpharmatics LLC
InventorsJohn Castle, Christopher K. Raymond, Christopher Armour
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The present invention provides methods for selectively amplifying a target population of nucleic acid molecules (e.g., all mRNA molecules expressed in a cell type except for the most highly expressed mRNA species). The present invention also provides populations of oligonucleotides including the nucleic acid sequences set forth in SEQ ID NOS:1-933. These oligonucleotides can be used, for example, to prime the synthesis of cDNA molecules complementary to mRNA molecules isolated from mammalian blood without priming the synthesis of cDNA molecules complementary to globin mRNA, or ribosomal RNA molecules.
A method of selectively amplifying a target population of nucleic acid molecules, the method comprising the step of using a population of oligonucleotides to prime the amplification of a target population of nucleic acid molecules within a larger population of nucleic acid molecules, wherein:
A method of selectively amplifying a target population of nucleic acid molecules, the method comprising the step of using a population of oligonucleotides to prime the amplification of a target population of nucleic acid molecules within a larger population of nucleic acid molecules, wherein:
A method of selectively amplifying a target population of nucleic acid molecules, the method comprising the step of using a population of oligonucleotides to prime the amplification of a target population of nucleic acid molecules within a larger population of nucleic acid molecules, wherein each oligonucleotide within the population of oligonucleotides comprises a hybridizing portion and a defined sequence portion located 5′ to the hybridizing portion, wherein the hybridizing portion is a member of the population of oligonucleotides comprising SEQ ID NOS:1-933.
A population of oligonucleotides comprising SEQ ID NOS:1-933.
A population of oligonucleotides consisting of SEQ ID NOS:1-933.
A population of oligonucleotides comprising at least 10% of the oligonucleotides comprising SEQ ID NOS:1-933.
A reagent for selectively amplifying a target population of nucleic acid molecules, the reagent comprising at least 10% of the oligonucleotides comprising SEQ ID NOS:1-933.
A reagent for selectively amplifying a target population of nucleic acid molecules, the reagent comprising a population of oligonucleotides to prime the amplification of a target population of nucleic acid molecules, wherein each oligonucleotide comprises a hybridizing portion and a defined sequence portion located 5′ to the hybridizing portion, wherein the hybridizing portion is a member of the population of oligonucleotides comprising SEQ ID NOS:1-933.
A kit for selectively amplifying a target population of nucleic acid molecules, the kit comprising a reagent comprising a population of oligonucleotides, wherein each oligonucleotide comprises a hybridizing portion that is a member of the population of oligonucleotides comprising SEQ ID NOS:1-933.
A method of selectively amplifying a target population of nucleic acid molecules, the method comprising the steps of:
A method of selectively amplifying a target population of nucleic acid molecules, the method comprising the steps of:
Dependencies are inferred from the claim text.