Sulfotransferase, peptide thereof and dna encoding the same
AssigneeSeikagaku Kogyo Kabushiki Kaisha
InventorsHisashi Narimatsu, Shigemi Sugioka, Hideo Mochizuki
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Abstract
A glycosaminoglycan sulfotransferase, a peptide thereof, a nucleic acid comprising a nucleotide sequence encoding the same, an enzyme agent for the synthesis of a glycosaminoglycan, which comprises the above-described enzyme or polypeptide, and a process for producing a glycosaminoglycan, which uses the enzyme agent.
Claims11
1-5. (canceled)
A nucleic acid selected from the group consisting of (I), (II) and (III):
- (I) a nucleic acid which encodes:
- a polypeptide which comprises amino acid numbers 37 to 346 in the amino acid sequence of SEQ ID NO:2, or
- a sulfotransferase which comprises a polypeptide which comprises amino acid numbers 37 to 346 in the amino acid sequence of SEQ ID NO:2 and has activity of transferring a sulfate group from a sulfate group donor to a glycosaminoglycan which is a sulfate group acceptor;
- (II) a nucleic acid which consists of the nucleotide sequence of SEQ ID NO:1 and
- (III) a nucleic acid which hybridizes, under stringent conditions, with:
- the nucleic acid according to (I) or (II) or a nucleic acid which consists of the nucleotide sequence of SEQ ID NO:1 or
- a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence of the nucleic acid according to (I) or (II) or the nucleotide sequence of SEQ ID NO:1.
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A process for producing a glycosaminoglycan comprising the structure of the following formula (1), which comprises reacting an enzyme agent with heparin or heparan sulfate to transfer a sulfate group from a sulfate group donor to a sulfate group acceptor:
- wherein the enzyme agent comprises a polypeptide which comprises amino acid numbers 37 to 346 in the amino acid sequence of SEQ ID NO:2, or a sulfotransferase which comprises the polypeptide and has activity of transferring a sulfate group from a sulfate group donor to a glycosaminoglycan which is a sulfate group acceptor.
(canceled)
Dependencies are inferred from the claim text.