Multiplexed assays of cell migration
AssigneeMillipore Corporation
InventorsSimon Goldbard, Ilya Ravkin, Oren E. Beske
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Abstract
Systems, including methods, apparatus, compositions, and kits, for multiplexed assay of cell migration with subdivided cell holders and/or microplates.
Claims16
A method of performing a cell migration assay, comprising (1) placing a set of cells into an array of compartments, each compartment having a bottom wall defined by a porous matrix and side walls connected to the bottom wall to separate the compartments;
- (2) contacting the bottom wall of each compartment with fluid held by a corresponding array of sub-wells in a microplate well;
- and (3) sensing migratory cells of the set of cells, if any, disposed below the bottom wall of each compartment.
7-17. (canceled)
A sample holder for performing cell migration assays, comprising an array of compartments separated by side walls each having a lower region, a subset of the side walls including openings defined in their lower regions, the openings being configured to permit migration of cells between adjacent compartments and to restrict passive movement of the cells between the adjacent compartments.
A method of performing a cell migration assay, comprising:
- placing cells into an array of first compartments so that the cells can migrate into laterally disposed second compartments through openings in walls separating respective pairs of first and second compartments, the openings being configured to restrict passive movement of the cells into the second compartments;
- and sensing migration of the cells to the second compartment, if any.
Dependencies are inferred from the claim text.