Method for the detection of proteolytic enzymes
AssigneeNederlandse Organisatie voor toegepast-natuurwetenschappelijk onderzoek TNO
InventorsJohan Hendrikus Verheijen, Jan Roeland Occo Hanemaaijer
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Abstract
The present invention provides an improved protease assay in which the proteases are detected on basis of their capability to cleave a modified pro-caspase which will yield an activated caspase which can then further be detected. Also part of the invention are the modified pro-caspases and kits comprising said modified pro-caspases.
Claims7
A modified pro-caspase derived from a pro-caspase by providing it with a recognition site which is cleavable by a protease different from the one which cleaves and activates the unmodified pro-caspase.
A kit for determining a protease, or its precursor after activation, in a sample, comprising a modified pro-caspase according to any of claims 1-3 or 5-7.
Dependencies are inferred from the claim text.