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US 2008/0187912 A1Published application
Oligonucleotide arrays for high resolution HLA typing
AssigneesFred Hutchinson Cancer Research Center, The University of Washington Office of Technology Transfer
InventorsEffie W. Petersdorf, Zhen Guo, John A. Hansen, Leroy Hood
Life of this patent
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Abstract
Arrays of HLA Class I oligonucleotide probes on a solid support are provided, wherein the probes are sufficient to represent at least 80% of the known polymorphisms in exons 2 and 3 of the HLA Class I locus.
Claims8
A method of HLA-B tissue typing, said method comprising:
- (a) amplifying exons 2 and 3 from a genomic sample of tissue using labeled primers and an asymmetric PCR method to form a labeled, single-stranded DNA sample;
- (b) contacting said labeled, single-stranded DNA sample with 4 an array of HLA-B oligonucleotide probes under hybridization conditions;
- and
- (c) detecting hybridization for said DNA sample and assigning an HLA-B allele type by analysis of said hybridization.
1 more element
Dependencies are inferred from the claim text.