Metal chelate-labelled pepetides
AssigneeRoche Diagnostics GmbH
InventorsChristoph Seidel, Ursula-Henrike Wienhues, Eva Hoss
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Abstract
The invention concerns a process for the production of metal chelate-labelled peptide antigens, peptides obtainable by this process and their use in an immunological method of detection.
Claims26
Metal chelate-labelled peptide which has a maximum length of 50 amino acids and is coupled to at least one luminescent metal chelate at least one amino side group, wherein the at least one luminescent metal chelate is present on the peptide at a predetermined position on the peptide, and wherein the peptide comprises an immunologically reactive epitope region derived from amino acid sequences of HCV.
A composition consisting essentially of metal chelate-labelled peptides each having a maximum length of 50 amino acids, wherein at least one luminescent metal chelate is coupled to each peptide at predetermined position(s) on the amino terminus and/or on amino side groups of the peptide, and wherein the composition is essentially free of peptides which have a luminescent metal chelate coupled at any position other than the predetermined position(s), and wherein the metal chelate-labelled peptides each comprise an immunologically reactive epitope region and the epitope region derived from amino acid sequences of HCV.
A metal-chelate labelled peptide which has a maximum length of 50 amino acids and is coupled to at least one luminescent metal chelate at the amino terminus and/or amino side groups, wherein the at least one luminescent metal chelate is present at predetermined position(s) on the peptide, and wherein the peptide comprises an immunologically reactive epitope region derived from amino acid sequences of HCV and the metal-chelate labelled peptide is prepared by one of the following processes:
- (1) synthesizing the peptide on a solid phase by attaching together a plurality of amino acids and/or amino acid derivatives;
- providing protecting groups on any amino side groups of the amino acids and/or amino acid derivatives to which coupling with the at least one luminescent metal chelate is to be avoided so that coupling of the at least one luminescent metal chelate in the following step may only proceed at the amino terminus of the peptide;
- and
- coupling the at least one luminescent metal chelate to the amino terminus, or
- (2) synthesizing the peptide on a solid phase by attaching together a plurality of amino acids and/or amino acid derivatives which are coupled to a luminescent metal chelate, wherein the amino acid derivatives are introduced at the predetermined position(s) on the peptide.
Dependencies are inferred from the claim text.