Method and Apparatus for Pressure Sodding a Biological Vascular Conduit
OwnerPaul J. Dimuzio, Patrick J. Greaney +1inventors
InventorsPaul J. Dimuzio, Patrick J. Greaney, Christopher A. Dimatteo
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Abstract
The present invention is directed to a method for lining a biological vascular conduit with cells. The method utilizes a suitable biologic tube conduit with luminal characteristics that simulate exposed basement membrane to allow for cell attachment. The biologic conduit is secured within a seeding chamber. Cells are introduced into the conduit. Pressure is applied to the seeding chamber such that each end receives substantially equal pressure.
Claims8
A method for lining a biologic vascular conduit with cells comprising the steps of:
- (a) utilizing a suitable biologic tube conduit with luminal characteristics that simulate exposed basement membrane to allow for cell attachment;
- (b) securing the biologic conduit within a seeding chamber;
- (c) introducing cells into the conduit;
- and
- (d) pressuring the seeding chamber at both ends, such that each end receives substantially equal pressure.
An apparatus for pressure sodding a biologic vascular conduit comprising:
- at least one graft treatment chamber having opposed ends;
- an attachment member at each end of the treatment chamber to secure a biologic tube conduit within said treatment chamber;
- and
- an adjustable pressure source located at each end of the treatment chamber,
- wherein substantially equal pressure can be applied at each end of the conduit.
Dependencies are inferred from the claim text.