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AssigneeUniversite Joseph Fourier
InventorsBenoit Polack, Bertrand Toussaint, Lauriane Quenee
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The present invention provides a vector for expression of a chimeric protein which is the result of the fusion between the N-terminal end of ExoS of Pseudomonas aeruginosa and an amino acid sequence of interest, the N-terminal end of ExoS corresponding to the N-terminal end of the chimeric protein. The vector contains the following from 5′ to 3′: a promoter, a nucleic acid comprising a nucleotide sequence encoding at least the 48 (SEQ ID 4) and at the most the 96 (SEQ ID 6) amino acids of the N-terminal end of ExoS; or any sequence of the same size that is at least 70% identical thereto and that has a secretion activity at the same level as that of ExoS, a nucleic acid comprising a nucleotide sequence encoding the amino acid sequence of interest.
A vector for expression of a chimeric protein whose N-terminal end corresponds to the fusion between the N-terminal end of ExoS of Pseudomonas aeruginosa and an amino acid sequence of interest, the N-terminal end of ExoS corresponding to the N-terminal end of the chimeric protein, said vector comprising the following from 5′ to 3′:
A cloning vector comprising from 5′ to 3′:
A chimeric protein whose N-terminal end comprises at least the 48 (SEQ ID 4) and at the most the 96 (SEQ ID 6) amino acids of the N-terminal end of ExoS of P. aeruginosa or any sequence of the same size, that is at least 70% identical thereto and that has a secretion activity at the same level as that of ExoS.
Dependencies are inferred from the claim text.