Cardiomyocyte Production
AssigneeES Cell International PTE Ltd.
InventorsBruce Paul Davidson, Ralph Eberhard Graichen, Robert Zweigerdt, Xiuqin Xu
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Abstract
The present invention provides a process of differentiating stem cells, in particular hES cells, into cardiomyocytes and into neural progenitors by growing the hES cells in the presence of a defined medium that is substantially free of xeno- and serum-components and thus comprises a clinically compliant medium. The defined media comprises defined factors that contribute to the promotion of differentiation to cardiomyocytes and neural progenitors. The invention also includes defined culture media and cell populations and methods of using them.
Claims67
A method of inducing or enhancing the induction of differentiation of stem cells into embryonic germ layer derivatives said method comprising culturing the stem cells in the presence of a defined medium that is substantially free of xeno- and serum-components said defined media comprising defined factors that contribute to the promotion of differentiation to embryonic germ layer derivatives.
A defined culture media when used for differentiating stem cells into embryonic germ layer derivatives which is substantially free of xeno- and serum-components said defined media comprising defined factors that contribute to the promotion of differentiation to embryonic germ layer derivatives.
A cell population comprising an enhanced proportion of an embryonic germ layer derivative that has selectively differentiated in a defined medium that is substantially free of xeno- and serum-components said defined medium comprising defined factors that selectively promote the differentiation of stem cells to the embryonic germ layer derivative.
Dependencies are inferred from the claim text.