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US 2008/0167213 A1Published application
Subtilase Variants
AssigneeNovozymes A/S
InventorsTina Sejersgard Fano, Claus Von Der Osten, Malene Kappen Krugen, Mads Norregaard-Madsen
Life of this patent
Estimating the term…
Abstract
The present invention relates to novel subtilase variants exhibiting alterations relative to the parent subtilase in one or more properties including: Wash performance, thermal stability, storage stability or catalytic activity. The variants of the invention are suitable for use in e.g. cleaning or detergent compositions, such as laundry detergent compositions and dishwash compositions, including automatic dishwash compositions.
Claims17
A subtilase variant comprising at least one or more of the following alterations:
- X11{A,R,N,D,C,Q,E,G,H,I,L,K,M,F,S,T,W,Y,V}, deletion, insertion;
- X30{A,R,N,D,C,Q,E,G,H,I,L,K,M,F,P,S,T,W,Y,V}, deletion, insertion;
- X31{R,N,D,Q,E,H,K,M,P,W,Y}, deletion, insertion;
- X32{A,R,D,C,E,G,H,I,L,K,M,F,P,T,W,Y,V}, insertion;
- X34{A,R,N,D,C,Q,E,G,H,I,L,K,M,F,S,T,W,Y,V}, deletion;
- X65{A,R,C,G,H,I,L,K,M,F,T,W,Y,V}, deletion, insertion;
- X66{A,R,C,H,I,L,K,M,F,T,W,Y,V}, deletion, insertion;
- X67{A,R,N,Q,G,H,I,K,M,F,P,S,T,W,Y,V};
- X68{R,N,D,Q,E,G,H,I,L,K,F,P,S,T,W,Y,V}, deletion, insertion;
- X69{A,R,N,D,Q,E,G,H,I,L,K,M,F,P,S,T,W,Y,V}, deletion, insertion;
- X70G;
- X71T;
- X77N;
- X83G, insertion;
- X84V;
- X85{A,R,N,D,Q,E,G,H,I,L,M,F,S,T,W,Y,V};
- X90{A,R,N,D,C,Q,E,G,H,I,L,K,M,F,P,S,T,W,Y,V}, deletion, insertion;
- X95{R,K,F,W,Y,V}, deletion;
- X107I
- X110G, insertion;
- X121V, insertion;
- X122 insertion;
- X123N;
- X125S;
- X150{A,R,N,D,Q,E,G,H,I,L,K,M,F,P,S,T,W,Y,V}, deletion, insertion;
- X152{A,R,N,D,Q,E,H,K,F,P,W,Y,V};
- X153{R,N,D,Q,E,G,H,I,L,K,M,F,T,W,Y,V};
- X154{A,R,G,H,I,L,M,F,W,Y,V};
- 164{A,R,H,T,W};
- X165{R,L,F,W,Y};
- X166{W,Y}, insertion;
- X175{R,N,D,Q,E,G,H,K,M,F,P,T,W,Y,V};
- X177{R,N,D,Q,E,G,H,I,L,K,M,F,P,S,T,W,Y,V}, deletion, insertion;
- X178{A,R,N,D,C,Q,E,G,H,I,L,K,M,F,P,S,T,W,Y,V}, deletion, insertion;
- X180{A,R,N,D,C,Q,E,G,H,I,L,K,M,F,P,S,T,W,Y,V}, deletion, insertion;
- X199{R,L,K,F,W,Y,V}, deletion, insertion;
- X200{A,R,I,L,K,M,F,W,Y,V}, deletion, insertion;
- X201{A,R,H,I,L,K,M,F,P,T,W,Y,V}, deletion, insertion;
- X202{A,R,C,G,H,I,L,K,M,F,T,W,Y,V};
- X207{A,R,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V};
- X220T, insertion;
- X223A, insertion;
- X225P, insertion;
- X226H, insertion;
- X227V, insertion;
- X228A, insertion;
- X229G, insertion;
- X230 insertion;
- X231A, insertion;
- X253{A,R,N,D,Q,E,G,H,I,L,M,F,S,T,W,Y,V}, deletion, insertion;
- X264{A,R,N,Q,G,H,I,L,M,F,S,T,W,Y,V}, deletion, insertion;
- X266{A,R,N,D,C,Q,E,G,H,I,L,K,M,F,S,T,W,Y,V}, deletion, insertion;
- wherein
- (a) the alteration(s) are independently, as specified,
- (i) a substitution of the amino acid that occupies the position with a different amino acid,
- (ii) a deletion of the amino acid which occupies the position, or
- (iii) an insertion of at least one additional amino acid downstream of the amino acid that occupies the position,
- (b) the variant has protease activity, and
- (c) each position corresponds to a position of the amino acid sequence of subtilisin BPN′, shown in FIG. 1.
56 more elements
Dependencies are inferred from the claim text.