Generation of Plants with Altered Oil Content

A method of producing a high oil phenotype in a plant, said method comprising:

• a) introducing into progenitor cells of the plant a plant transformation vector comprising a nucleotide sequence that encodes or is complementary to a sequence that encodes a HIO polypeptide comprising the amino acid sequence of SEQ ID NO:
• 2 or 4, or an ortholog thereof, and
• b) growing the transformed progenitor cells to produce a transgenic plant, wherein said polynucleotide sequence is expressed, and said transgenic plant exhibits an altered oil content phenotype relative to control plants.

A method of generating a plant having a high oil phenotype comprising identifying a plant that has an allele or mutation in its HIO103.1 or HIO101B nucleic acid sequence that results in increased oil content, compared to plants lacking the allele, and generating progeny of said identified plant, wherein the generated progeny inherit the allele and have the high oil phenotype.

A feed, meal, grain, food, or seed comprising a polypeptide encoded by the nucleic acid sequence as set forth in SEQ ID NO:

• 1 or 3.

A feed, meal, grain, food, or seed comprising a polypeptide comprising an amino acid sequence as set forth in SEQ ID NO:

• 2 or 4, or an ortholog thereof.