Method of providing readily available cellular material derived from peripheral blood, and a composition thereof
OwnerDavid Wolf, Donnie Ruddinventors
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Abstract
The present invention is directed to the process of preparing an expanded peripheral blood stem cell composition, preparing a TVEMF-expanded peripheral blood stem cell composition, methods of producing the same, and to repair of tissue and/or function with expanded, and TVEMF-expanded, peripheral blood stem cells.
Claims45
Time varying electromagnetic force (“TVEMF”) expanded peripheral blood stem cells from a mammal,
- wherein the expanded peripheral blood stem cells have been TVEMF-expanded in a three-dimensional environment of a TVEMF-bioreactor rotating about its horizontal longitudinal central axis;
- wherein the expanded peripheral blood stem cells are genetic expression modified by the TVEMF-expansion in the TVEMF bioreactor;
- and
- wherein the cells are expanded in the TVEMF-bioreactor without substantial differentiation.
A process for preparing a peripheral blood stem cell composition comprising the steps of:
- a. placing a peripheral blood mixture comprising peripheral blood stem cells in a culture chamber of a rotatable TVEMF-bioreactor comprising a TVEMF source to establish a three-dimensional culture;
- b. rotating the rotatable TVEMF-bioreactor comprising a coil about its horizontal longitudinal central axis to suspend the cells without substantial differentiation and wherein the cells are genetic expression modified according to the three-dimensional culture;
- and
- c. subjecting cells to a TVEMF and TVEMF-expanding the peripheral blood stem cells in the TVEMF-bioreactor to prepare the peripheral blood stem cell composition.
A method of researching a disease state comprising introducing a TVEMF-expanded stem cell into a test system for the disease state.
Bone marrow stem cells from a mammal, wherein said bone marrow stem cells are TVEMF-expanded.
A process for preparing an expanded peripheral blood stem cell composition comprising the steps of:
- placing a peripheral blood stem cell mixture comprising peripheral blood stem cells in a rotatable bioreactor;
- expanding the peripheral blood stem cells by rotating the bioreactor about a substantially horizontal vertical central axis to suspend the cells in the rotating bioreactor;
- preparing an expanded peripheral blood stem cell composition with the expanded peripheral blood stem cells.
Dependencies are inferred from the claim text.