Method for isolating and/or identifying mesenchymal stem cells (msc)
AssigneeEberhard-Karls-Universitat Tuebingen Universitaetsklinikum
InventorsHans-Jorg Buhring, Reiner Lammers, Sabrina Treml, Venkata Battula
Life of this patent
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Abstract
The present invention concerns antibodies produced from hybridoma cell lines chosen from the group comprising W8B2, W1C3, W7C6, W5C4, 24D2, 28D4, HEK-3D6, W4A5, W3D5, W5C5, 9A3G9, 58B1, F9-3C2F1, 39D5, for isolating and/or identifying homogenous mesenchymal stem cells. Furthermore a method is presented with which mesenchymal stem cells from adult primary tissue, for example bone marrow, can be identified and isolated with a high level of purity.
Claims13
A method for isolating and/or identifying homogenous mesenchymal stem cells from primary tissue, which includes the following steps:
- a) putting a sample suspension of cells obtained from primary tissue and including mesenchymal stem cells in contact with an antibody or functional fragments thereof, produced from hybridoma cell lines selected from the group consisting of W8B2, W1C3, W7C6, W5C4, 24D2, 28D4, HEK-3D6, W4A5, W3D5, W5C5, 9A3G9, 58B1, F9-3C2F1, 39D5, and
- b) identification and/or isolation of those cells in the sample to which an anti-body from step a), or fragments thereof have bound.
A method for isolating and/or identifying homogenous mesenchymal stem cells from primary tissue, which includes the following steps:
- a) putting a sample suspension of cells obtained from primary tissue and including mesenchymal stem cells in contact with an anti-CD271 antibody;
- b) putting the sample from step a) in contact with an antibody or functional fragments thereof, produced from hybridoma cell lines selected from the group consisting of W8B2, W1C3, W7C6, W5C4, 24D2, 28D4, HEK-3D6, W4A5, W3D5, W5C5, 9A3G9, 58B1, F9-3C2F1, 39D5;
- and
- c) identification and/or isolation of those cells in the sample to which the antibody from step a), and an antibody from step b) or functional fragments thereof have bound.
A method for isolating and/or identifying homogenous mesenchymal stem cells from primary tissue, which includes the following steps:
- a) putting a sample suspension of cells obtained from primary tissue and including mesenchymal stem cells in contact with an antibody or functional fragment thereof, that binds to the same antigen as an antibody produced from the hybridoma cell lines W8B2, W1C3, W7C6, W5C4, 24D2, 28D4, HEK-3D6, W4A5, W3D5, W5C5, 9A3G9, 58B1, F9-3C2F1, 39D5;
- b) identification and/or isolation of those cells in the sample to which an anti-body from step a), or fragments thereof have bound.
A pharmaceutical composition containing an antibody or functional fragments thereof, produced from a hybridoma cell line selected from the group consisting of W1C3, W7C6, W5C4, 24D2, 28D4, HEK-3D6, W4A5, W3D5, W5C5, 9A3G9, 58B1, F9-3C2F1, 39D5.
A kit containing at least one antibody or functional fragments thereof, produced from a hybridoma cell line selected from the group consisting of W1C3, W7C6, W5C4, 24D2, 28D4, HEK-3D6, W4A5, W3D5, W5C5, 9A3G9, 58B1, F9-3C2F1, 39D5.
An antibody, or functional fragments thereof, produced from one of hybridoma cell lines W8B2, W1C3, W7C6, W5C4, 24D2, 28D4, HEK-3D6, W4A5, W3D5, W5C5, 9A3G9, 58B1, F9-3C2F1, 39D5, for producing a medicinal product for gene or cell therapy.
An antibody, or functional fragments thereof, produced from one of hybridoma cell lines W8B2, W1C3, W7C6, W5C4, 24D2, 28D4, HEK-3D6, W4A5, W3D5, W5C5, 9A3G9, 58B1, F9-3C2F1, 39D5, for treating deteriorated bone or cartilage tissue.
Method for producing osteoblasts, chondrocytes, adipocytes or fibroblasts in vitro, comprising the following steps:
- a) putting a sample suspension of cells obtained from primary tissue and including mesenchymal stem cells in contact with an antibody or functional fragments thereof, produced from hybridoma cell lines selected from the group consisting of W8B2, W1C3, W7C6, W5C4, 24D2, 28D4, HEK-3D6, W4A5, W3D5, W5C5, 9A3G9, 58B1, F9-3C2F1, 39D5, and
- b) identification and/or isolation of those cells in the sample to which an anti-body from step a), or fragments thereof have bound;
- c) cultivating the cells isolated and/or identified in step b) under conditions wherein said cells differentiate into osteoblasts, chondrocytes, adipocytes or fibroblasts.
Dependencies are inferred from the claim text.