Observational liquid/gas environment combined with specimen chamber of electron microscope

An observational liquid/gas environment combined with a specimen chamber of an electron microscope, said electron microscope having two pole pieces defined as an upper pole piece and a lower pole piece mounted at an inner upper side thereof and an inner lower side thereof respectively, an electron beam through tunnel being formed at a center of each of said two pole pieces for penetration of said electron beam, said two pole pieces being spaced from each other for a predetermined interval, said specimen chamber being located between said two pole pieces, said environment comprising:

• at least two buffer chambers formed by a plurality of spacers in cooperation with said two pole pieces, said buffer chambers being located at an upper side and a lower side of said specimen chamber respectively and spaced from each other for a predetermined interval, one of said buffer chambers extending into and overlapping said specimen chamber in space, said spacer having an inner aperture located at an end of each of said buffer chambers and close to said specimen chamber, said spacer having an outer aperture located at an end of each of said buffer chambers and away from said specimen chamber, said inner and outer apertures being coaxially aligned with one another and crossing a path that said electron beam of said electron microscope passes, each of said buffer chambers being connected with a gas-pumping source;
• and
• a gas source connected with said specimen chamber for providing a gas and keeping a predetermined gas pressure of said specimen chamber;
• wherein the distance between said inner apertures is smaller than that of said two pole pieces, and said spacers having said inner apertures are located in said specimen chamber or said electron beam through tunnel.