Steroid responsive nucleic acid expression and prediction of disease activity
AssigneeExpression Diagnostics, Inc.
InventorsPreeti Lal, Steven Rosenberg, Tod Klinger
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Abstract
The invention relates to methods useful for diagnosing and monitoring the steroid responsiveness of a subject by detecting expression of steroid modulated genes and for predicting transplant rejection and non-rejection.
Claims39
A method of diagnosing or monitoring steroid responsiveness of a subject comprising:
- a) detecting expression of a diagnostic set of at least two steroid modulated nucleic acids in a sample from the subject wherein the expression is correlated with steroid administration or dosage;
- and
- b) applying at least one statistical method to the expression of the diagnostic set to diagnose or monitor steroid responsiveness of the subject.
A kit comprising the oligonucleotides or probe sets of claim 13.
A method for predicting rejection or non-rejection in a subject with a transplant comprising:
- a) detecting expression of a diagnostic set of at least two steroid modulated nucleic acids in a sample from the subject wherein the expression of the steroid modulated nucleic acids correlates with transplant rejection or non-rejection;
- and
- b) applying at least one statistical method to the expression of the diagnostic set of steroid modulated nucleic acids to predict rejection or non-rejection.
A method of diagnosing or monitoring the status of a subject with a transplant comprising:
- a) detecting expression of a diagnostic set of at least two steroid modulated nucleic acids in a sample from the subject wherein the expression is correlated with dysfunction or rejection of the transplant;
- and
- b) applying at least one statistical method to the expression of the nucleic acids to monitor the status of the transplant.
A method for designing and monitoring a treatment plan for a subject with a transplant or an immune disorder comprising:
- a) detecting expression of a diagnostic set of at least two steroid modulated nucleic acids in a sample from the subject wherein the expression correlates with the steroid responsiveness of the subject;
- and
- b) using the expression of the diagnostic set of steroid modulated nucleic acids to design and monitor the treatment plan of the subject.
A method for using primers and probe sets to detect steroid responsiveness of a subject with a transplant or an immune disorder comprising:
- a) designing and generating primers or probe sets for nucleic acids whose expression is modulated by steroid administration or dosage;
- and
- b) using RT-PCR and the primers or probe sets on a sample from the subject to detect steroid responsiveness.
Dependencies are inferred from the claim text.