US 2007/0117085 A1 · Method and device for culturing live cells by coupling a bioreactor receiver with a selection automation

Abstract

The invention relates to a method for continuously, semi-continuously or discontinuously treating a substrate (24) consisting in placing said substrate in a bioreactor receiver (1) and in exposing it to the action of a living cell culture (C1) which makes it possible to carry out a reaction (R1) on the substrate (24) and to which a medium is periodically inoculated with the aid of living cells (C2) improving said reaction. Said living cells (C2) are obtainable by selection from a population of dynamic living cells carried out by an automatic selection device (2) which is supplied by the same substrate (24) as the bioreactor receiver (1) and is originally inoculated with the living cells (C1) contained in the bioreactor receiver (1) tank and an operating device.

Claims15

Dependent claims

1-12. (canceled)

Method for the continuous, semi-continuous or discontinuous treatment of a substrate (24), in which said substrate (24) installed in a bioreactor vessel (1) is subjected to the action of a culture of living cells C1 making it possible to carry out a reaction R1 on said substrate (24), characterized in that it comprises the following steps:

a) removing a population of living cells C1 present in the bioreactor vessel (1) in order to transfer them into an automatic device (2) for selecting living cells proliferating in suspension;
b) selecting the cells C2 proliferating in suspension in said device (2) by eliminating the static living cells, and
c) transferring all or part of cells C2 present in said selection device (2) periodically to the bioreactor vessel (1).

Device for culturing living cells characterized in that it comprises:

A:
a bioreactor vessel (1) capable of containing a population of living cells C1 carrying out a reaction R1 of bioconversion of a substrate (24);
B:
an automatic device (2) for selecting living cells proliferating in suspension, making it possible to select living cells C2 proliferating in suspension by elimination of the static living cells, this device comprising a set of means making it possible to supply of said device with sterilization, washing or neutralizing liquids;
C:
a system of pipes (5) comprising means for carrying out transfers from the selection device (2) to the bioreactor vessel (1);
and
D:
a system of pipes (6) comprising means for carrying out transfers from the bioreactor vessel (1) to the selection device (2).

6 more elements

Dependencies are inferred from the claim text.

Facts

Application

10/574,197Filed 30 Sep 2004

Priority

FR 03115021 Oct 2003

Published

24 May 2007

Term ends

Not estimatedThe source records don’t support an estimate.

Classification14 codes

C12M 23/58
Constructional details, e.g. recesses, hinges: reaction vessels connected in series or in parallel
C02F 3/348
Characterised by the microorganisms used: characterised by the way or the form in which the microorganisms are added or dosed
C12M 29/18
Means for introduction, extraction or recirculation of materials, e.g. pumps: external loop; Means for reintroduction of fermented biomass or…
C12M 33/22
Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus:…

Method and device for culturing live cells by coupling a bioreactor receiver with a selection automation

Life of this patent

Method and device for culturing live cells by coupling a bioreactor receiver with a selection automation

Life of this patent

Abstract

Claims15