Compounds targeted to cellular locations
AssigneeAdProTech Limited
InventorsPamela Rowling, Geoffrey Smith, Simon Ridley
Life of this patent
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Abstract
A soluble derivative of a soluble polypeptide, which comprises two or more heterologous membrane binding elements with low membrane affinity covalently associated with the polypeptide, the elements being soluble in aqueous solution, and the elements being capable of interacting, independently and with themodynamic additivity, with components of cellular or artificial membranes exposed to extracellular fluids, characterized in that the membrane binding elements target lipid raft components of the membrane and bind to the lipid rafts to localize the polypeptide at the lipid rafts.
Claims18
A compound comprising a membrane binding peptide, wherein the peptide is modified by the addition of a C-terminal cysteine residue, wherein the C-terminal cysteine residue is covalently associated with a fluorescein derivative which is covalently associated through a S-5-succinimido group, and wherein the compound is selected from the group consisting of:
- a) N-Myristoyl-Gly-Ser-Ser-Lys-Ser-Pro-Ser-Lys-Lys-Lys-Lys-Lys-Lys-Pro-Gly-Glu-Cys(S-5-succinimido fluorescein)-NH2 (APT2087) (SEQ ID NO:
- 13),
- b) N-Myristoyl-Gly-Ser-Ser-Lys-Ser-Pro-Ser-Lys-Lys-Lys-Lys-Lys-Lys-Pro-Gly-Glu-Cys(S-5-succinimido Alexafluor™488 C5)-NH2 (APT2104) (SEQ ID NO:
- 14), and
- c) N-Myristoyl-Gly-Ser-Ser-Lys-Ser-Pro-Ser-Lys-Lys-Lys-Lys-Lys-Lys-Pro-Gly-Glu-Cys(S-5-succinimido Alexafluor™546 C5)-NH2 (APT2105) (SEQ ID NO:
- 15).
16-19. (canceled)
21-22. (canceled)
Dependencies are inferred from the claim text.