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US 2006/0234321 A1Published application
Syphilis diagnostic tests and kits
AssigneeUniversity of Washington
InventorsWesley Van Voorhis, Caroline Cameron, Sheila Lukehart, Janelle Kuroiwa
Life of this patent
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Abstract
The present invention provides methods and kits for determining whether a human subject is infected with T. pallidum, and isolated fragments of T. pallidum protein Tp92 that can be used in the methods and kits of the invention.
Claims20
A method for determining whether a human subject is infected with T. pallidum, the method comprising the steps of:
- (a) contacting an isolated polypeptide selected from the group consisting of a fragment of at least twelve consecutive amino acids of a Tp92 protein, a fragment of at least twelve consecutive amino acids of a Tp0453 protein, and a fragment of at least twelve consecutive amino acids of a Gpd protein, with antibodies from a human subject;
- and
- (b) determining whether the antibodies bind to the isolated polypeptide, wherein binding of the antibodies to the isolated polypeptide indicates that the human subject is infected with T. pallidum.
A kit comprising:
- (a) a substrate comprising an isolated polypeptide selected from the group consisting of a fragment of at least twelve consecutive amino acids of a Tp92 protein, a fragment of at least twelve consecutive amino acids of a Tp0453 protein, and a fragment of at least twelve consecutive amino acids of a Gpd protein;
- (b) reagents for labeling antibody bound to the isolated polypeptide;
- and
- (c) written indicia providing a user with instructions for use of the kit to determine whether a human subject is infected with T. pallidum.
1 more element
An isolated polypeptide comprising an amino acid sequence selected from the group of amino acid sequences set forth in SEQ ID NOS:7-21.
Dependencies are inferred from the claim text.