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US 2006/0234298 A1Published application
Systems and methods for rapidly changing the solution environment around sensors
AssigneeCellectricon AB
InventorsDaniel Chiu, Owe Orwar, Kent Jardemark, Mattias Karlsson, Jessica Olofsson, Johan Pihl, Jon Sinclair
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Abstract
The invention provides microfluidic systems for altering the solution environment around a nanoscopic or microscopic object, such as a sensor, and methods for using the same. The invention also provides a system and methods for modulating, controlling, preparing, and studying receptors.
Claims58
A method for modulating, controlling, preparing, or studying receptors, comprising:
- a) providing a substrate, the substrate comprising:
- a chamber comprising a cell-based biosensor, the cell-based biosensor comprising a receptor which is activated by an agonist or a receptor which is inactivated by an antagonist;
- and
- a plurality of delivery channels delivering one or more of sample, agonist, antagonist, each channel comprising an outlet for delivering a substantially separate aqueous stream into the chamber;
- and
- (b) sequentially exposing the biosensor to a fluid stream from two or more outlets.
3 more elements
A method of preparing a receptor in a discrete kinetic state, comprising:
- sequentially exposing a cell-based biosensor to two or more concentrations of modulator, and
- alternating resting and washing periods between exposures to modulator, wherein the sequential exposure arrests the biosensor in a pre-determined kinetic state.
Dependencies are inferred from the claim text.