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US 2006/0233806 A1Published application
Removal promoters and inhibitor for apoptosis cells in vivo
OwnerShigekazu Nagatainventor
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OwnerShigekazu Nagatainventor
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The present invention is to provide a removal promoter for apoptotic cells which is capable of immediately removing apoptotic cells in vivo by macrophages, or a removal inhibitor which inhibits the removal of apoptotic cells in vivo by macrophages. A removal promoter for apoptotic cells in vivo containing the milk fat globule-EGF factor 8-L (MFG-E8-L), MFG-E8-L mutant having removal promotion action for apoptotic cells in vivo by macrophages, or preferably a recombinant human or mouse MFG-E8-L, or a recombinant human or mouse MFG-E8-L mutant as an active ingredient is prepared. Such removal promoters specifically bind to apoptotic cells and promote the phagocytosis of apoptotic cells by macrophages by recognizing aminophospholipids such as phosphatidylserine exposed on apoptotic cell surface. On the other hand, a point mutation (D89E) MFG-E8-L mutant is used as a removal inhibitor.
A removal promoter for apoptotic cells in vivo by macrophages which contains MFG-E8-L as an active ingredient.
A removal promoter for apoptotic cells in vivo by macrophages which is comprised of amino acid sequence wherein one or more amino acids are deleted, substituted or added in the amino acid sequence comprising MFG-E8-L, and which contains MFG-E8-L mutant having removal promotion action for apoptotic cells in vivo by macrophages as an active ingredient.
A removal inhibitor for apoptotic cells in vivo by macrophages comprised of amino acid sequence wherein one or more amino acids are deleted, substituted or added in the amino acid sequence comprising MFG-E8-L, and which contains a MFG-E8-L mutant having removal inhibition action for apoptotic cells in vivo by macrophages as an active ingredient.
A detection agent for apoptotic cells in vivo which contains a labeled MFG-E8-L or MFG-E8-L mutant having removal promotion action for apoptotic cells in vivo by macrophages, or an antibody against them, or a labeled MFG-E8-L mutant having removal inhibition action for apoptotic cells in vivo by macrophages as an active ingredient.
A detection method for apoptotic cells in vivo wherein a detection agent for apoptotic cells in vivo which contains a labeled MFG-E8-L or MFG-E8-L mutant having removal promotion action for apoptotic cells in vivo by macrophages, or an antibody against them, or a labeled MFG-E8-L mutant having removal inhibition action for apoptotic cells in vivo by macrophages as an active ingredient is used.
A screening method for a removal promotion inducing substance or a removal promotion suppressive substance for apoptotic cells in vivo by macrophages wherein MFG-E8-L or a MFG-E8-L mutant having removal promotion action for apoptotic cells in vivo by macrophages, or an antibody against them is contacted with a test substance, to evaluate the extent of removal of apoptotic cells in vivo.
A screening method for a removal inhibition inducing substance or a removal inhibition suppressive substance for apoptotic cells in vivo by macrophages wherein a MFG-E8-L mutant which has removal inhibition action for apoptotic cells in vivo by macrophages is contacted with a test substance, to evaluate the extent of removal inhibition of apoptotic cells in vivo.
Dependencies are inferred from the claim text.