Novel human polynucleotides and the polypeptides encoded thereby
AssigneeLexicon Genetics Incorporated
InventorsMichael Nehls, Brian Zambrowicz, Arthur Sands
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Abstract
Novel human polynucleotides are disclosed that correspond to human gene trapped sequences, or GTSs. The disclosed GTSs are useful for gene discovery and as markers for, inter alia, gene expression analysis, forensic analysis, and determining the genetic basis of human disease.
Claims9
A synthetic oligonucleotide comprising a contiguous stretch of at least about 15 nucleotides first disclosed in at least one of SEQ ID NOS:9-1008.
An isolated cDNA polynucleotide derived from the genome of a human that is capable of hybridizing to a sequence first disclosed in at least one of SEQ ID NOS:9-1008 under stringent conditions.
An isolated polynucleotide comprising a contiguous stretch of at least about 60 nucleotides first disclosed in at least one of SEQ ID NOS:9-1008.
An in vitro process for producing a polynucleotide comprising the steps of:
- a) obtaining a polynucleotide template encoding a sequence capable of hybridizing to a GTS of SEQ ID NOS:9-1008;
- b) combining said template with a synthetic oligonucleotide sequence of about 14 to about 80 bases in length that comprises a contiguous sequence of at least about 12 nucleotides disclosed in one of SEQ ID NOS:9-1008;
- and
- c) processing the combined oligonucleotide and template preparation such that said oligonucleotide sequence hybridizes to said template in the presence of a DNA polymerase molecule and a sufficient concentration of dNTPs for said oligonucleotide sequence to prime DNA synthesis by said polymerase,
- wherein a polynucleotide is produced that encodes at least about 50 contiguous nucleotides first disclosed in one of SEQ ID NOS:9-1008.
Dependencies are inferred from the claim text.