Postpartum-derived cells for use in treatment of disease of the heart and circulatory system

An isolated postpartum-derived cell comprising an L-valine-requiring cell derived from human postpartum tissue substantially free of blood, said cell capable of self-renewal and expansion in culture and having the potential to differentiate into a cell of cardiomyocyte phenotypes;

• the cell capable of growth in an atmosphere containing oxygen from about 5% to at least about 20%;
• wherein said cell comprises at least one of the following characteristics:
• potential for at least about 40 doublings in culture;
• attachment and expansion on a coated or uncoated tissue culture vessel, wherein a coated tissue culture vessel comprises a coating of gelatin, laminin, collagen, polyornithine, vitronectin, or fibronectin;
• production of at least one of tissue factor, vimentin, and alpha-smooth muscle actin;
• production of at least one of CD10, CD13, CD44, CD73, CD90, PDGFr-alpha, PD-L2 and HLA-A,B,C;
• lack of production of at least one of CD31, CD34, CD45, CD80, CD86, CD117, CD141, CD178, B7-H2, HLA-G, and HLA-DR,DP,DQ, as detected by flow cytometry;
• expression of at least one of interleukin 8;
• reticulon 1;
• chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha);
• chemokine (C-X-C motif) ligand 6 (granulocyte chemotactic protein 2);
• chemokine (C-X-C motif) ligand 3;
• and tumor necrosis factor, alpha-induced protein 3;
• expression of at least one of C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 2 (activation-induced);
• Wilms tumor 1;
• aldehyde dehydrogenase 1 family, member A2;
• and renin;
• oxidized low density lipoprotein (lectin-like) receptor 1;
• Homo sapiens, clone IMAGE:4179671, mRNA, partial cds;
• protein kinase C, zeta;
• hypothetical protein DKFZp564FO13;
• downregulated in ovarian cancer 1;
• Homo sapiens mRNA;
• and cDNA DKFZp547K1113 (from clone DKFZp547K1113);
• expression, which relative to a human cell that is a fibroblast, a mesenchymal stem cell, or an ileac crest bone marrow cell, is reduced for at least one of:
• short stature homeobox 2;
• heat shock 27 kDa protein 2;
• chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1);
• elastin (supravalvular aortic stenosis, Williams-Beuren syndrome);
• Homo sapiens mRNA;
• cDNA DKFZp586M2022 (from clone DKFZp586M2022);
• mesenchyme homeobox 2 (growth arrest-specific homeobox);
• sine oculis homeobox homolog 1 (Drosophila);
• crystallin, alpha B;
• dishevelled associated activator of morphogenesis 2;
• DKFZP586B2420 protein;
• similar to neuralin 1;
• tetranectin (plasminogen binding protein);
• src homology three (SH3) and cysteine rich domain;
• B-cell translocation gene 1, anti-proliferative;
• cholesterol 25-hydroxylase;
• runt-related transcription factor 3;
• hypothetical protein FLJ23191;
• interleukin 11 receptor, alpha;
• procollagen C-endopeptidase enhancer;
• frizzled homolog 7 (Drosophila);
• hypothetical gene BC008967;
• collagen, type VIII, alpha 1;
• tenascin C (hexabrachion);
• iroquois homeobox protein 5;
• hephaestin;
• integrin, beta 8;
• synaptic vesicle glycoprotein 2;
• Homo sapiens cDNA FLJ12280 fis, clone MAMMA1001744;
• cytokine receptor-like factor 1;
• potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4;
• integrin, alpha 7;
• DKFZP586L151 protein;
• transcriptional co-activator with PDZ-binding motif (TAZ);
• sine oculis homeobox homolog 2 (Drosophila);
• KIAA1034 protein;
• early growth response 3;
• distal-less homeobox 5;
• hypothetical protein FLJ20373;
• aldo-keto reductase family 1, member C3 (3-alpha hydroxysteroid dehydrogenase, type II);
• biglycan;
• fibronectin 1;
• proenkephalin;
• integrin, beta-like 1 (with EGF-like repeat domains);
• Homo sapiens mRNA full length insert cDNA clone EUROIMAGE 1968422;
• EphA3;
• KIAA0367 protein;
• natriuretic peptide receptor C/guanylate cyclase C (atrionatriuretic peptide receptor C);
• hypothetical protein FLJ 14054;
• Homo sapiens mRNA;
• cDNA DKFZp564B222 (from clone DKFZp564B222);
• vesicle-associated membrane protein 5 (myobrevin);
• EGF-containing fibulin-like extracellular matrix protein 1;
• BCL2/adenovirus EIB 19 kDa interacting protein 3-like;
• AE binding protein 1;
• cytochrome c oxidase subunit VIIIa polypeptide 1 (muscle);
• neuroblastoma, suppression of tumorigenicity 1;
• insulin-like growth factor binding protein 2, 36 kDa;
• secretion of at least one of MCP-1, IL-6, IL-8, GCP-2, HGF, KGF, FGF, HB-EGF, BDNF, TPO, MIP1a, RANTES, and TIMP1;
• and
• lack of secretion of at least one of TGF-beta2, ANG2, PDGFbb, MIP1b, 1309, MDC, and VEGF, as detected by ELISA.