Method for measuring the surface plasmon resonance

Abstract

It is an object of the present invention to provide a method for obtaining a chromatogram using the SPR analysis method. The present invention provides a method for measuring the change in the surface plasmon resonance by using a surface plasmon resonance measurement device which comprises a metal film, a light source for generating a light beam, an optical system for allowing such a light beam to enter the interface of the metal film so that total reflection conditions can be obtained at the interface thereof and so that various incidence angles can be included, a flow channel system comprising a cell formed on said metal film, and a light-detecting means for detecting the state of surface plasmon resonance by measuring the intensity of the light beam totally reflected at said interface, and exchanging a liquid contained in the flow channel system; wherein the measurement is carried out by supplying a liquid at a liquid amount such that the absolute amount of molecules adsorbed on the metal film surface is lower than the saturated adsorption amount of said molecules on said metal film surface.

Dependencies are inferred from the claim text.