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AssigneeJ.R. Simplot Company
InventorsCaius Rommens, Jingsong Ye, Jaime Menendez-Humara, Hua Yan, Craig Richael, W. Brinkerhoff, Kathy Swords
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The present invention relates to a new plant breeding process. The process improves the agronomic performance of crop plants by using genetic material that is also used in classical breeding. Instead of sexually recombining entire genomes at random, as is done in classical breeding, specific genetic elements are rearranged in vitro and inserted back into individual plant cells. Plants obtained through this new plant breeding process do not contain foreign nucleic acid but only contain nucleic acid from the plant species selected for transformation or plants that are sexually compatible with the selected plant species. Plants developed through this new plant breeding process are provided. In particular, potato plants displaying improved tuber storage and health characteristics are provided.
A method of transforming a plant cell with a desired polynucleotide, comprising contacting a plant cell with an Agrobacterium strain that comprises a first plasmid, wherein the first plasmid comprises (i) nucleic acid sequences obtained from at least one plant genome that facilitate enzymatic nicking of the plasmid in Agrobacterium and (ii) a desired polynucleotide, wherein any plant nucleic acid that facilitates enzymatic nicking of the plasmid is not an Agrobacterium T-DNA or an Agrobacterium T-DNA border sequence, and wherein a cell that comprises the desired polynucleotide in its genome is a transformed plant cell.
A nucleic acid sequence with a 5′-end and a 3′-end, wherein (a) the 5′-end comprises the consensus sequence of (i) 5′-YGRYAGGATATATWSNVBKGTAAWY-3′ (SEQ ID NO:
A plant transfer-DNA, comprising a sequence with at least 70% identity to at least part of the sequence of SEQ ID NOs:
Dependencies are inferred from the claim text.