Novel cytochrome P450 monooxygenase
OwnerBrian McGonigle, Saverio Falco +2inventors
InventorsBrian McGonigle, Saverio Falco, John Everard, Nicole Swinson
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Abstract
This invention relates to an isolated polynucleotide encoding a cytochrome P450 monooxygenase that in the presence of linoleic acid results in production of 1-octen-3-ol. The invention also relates to the construction of a recombinant DNA construct comprising all or a portion of the polynucleotide encoding the cytochrome P450 monooxygenases of the invention, in sense or antisense orientation, wherein expression of the recombinant DNA construct results in production of altered levels 1-octen-3-ol in a transformed host cell.
Claims37
An isolated polynucleotide comprising:
- (a) a nucleotide sequence encoding a cytochrome P450 polypeptide having an amino acid sequence of at least 80% sequence identity, based on the Clustal V method of alignment, when compared to an amino acid sequence of SEQ ID NO:3 , wherein expression of said polypeptide in an appropriate host cell transformed with said isolated polynucleotide, in the presence of linoleic acid, results in an increased level of 1-octen-3-ol in said transformed host cell when compared to an nontransformed host cell;
- or
- (b) a complement of the nucleotide sequence, wherein the complement and the nucleotide sequence consist of the same number of nucleotides and are 100% complementary.
A portion of SEQ ID NOS:
- 2, 8, or 21 wherein said portion is capable of suppressing production of 1-octen-3-ol when introduced into cells that normally produce 1-octen-3-ol.
Dependencies are inferred from the claim text.