Expression cassettes for mesophyll- and/or epidermis-preferential expression in plants
AssigneeSunGene GmbH
InventorsUlrich Keetman, Karin Herbers, Helke Hillebrand
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Abstract
The present invention relates to expression cassettes comprising transcription regulating sequences with mesophyll- and/or epidermis-preferential or mesophyll- and/or epidermis-specific expression profiles in plants obtainable from Arabidopsis thaliana genes At5g13220, At1g68850, At4g36670, At3g10920, At1g33240, or At1g28440.
Claims12
An expression cassette for regulating mesophyll- and/or epidermis-preferential or mesophyll- and/or epidermis-specific expression in plants comprising
- i) at least one transcription regulating nucleotide sequence of a plant gene, said plant gene selected from the group of genes described by the GenBank Arabidopsis thaliana genome loci At5g13220, At1g68850, At4g36670, At3g10920, At1g33240, and At1g28440, or a functional equivalent thereof, and functionally linked thereto
- ii) at least one nucleic acid sequence which is heterologous in relation to said transcription regulating nucleotide sequence.
A method for identifying and/or isolating a sequence with mesophyll- and/or epidermis-preferential or mesophyll- and/or epidermis-specific transcription regulating activity characterized that said identification and/or isolation utilizes a nucleic acid sequence encoding a amino acid sequence as described by SEQ ID NO:
- 8, 18, 32, 42, 52, or 60 or a part of at least 15 bases thereof.
A method for providing a transgenic expression cassette for mesophyll- and/or epidermis-preferential or mesophyll- and/or epidermis-specific expression comprising the steps of:
- I. isolating of a mesophyll- and/or epidermis-preferential or mesophyll- and/or epidermis-specific transcription regulating nucleotide sequence utilizing at least one nucleic acid sequence or a part thereof, wherein said sequence is encoding a polypeptide described by SEQ ID NO:
- 8, 18, 32, 42, 52, or 60, or a part of at least 15 bases thereof, and
- II. functionally linking said mesophyll- and/or epidermis-preferential or mesophyll- and/or epidermis-specific transcription regulating nucleotide sequence to another nucleotide sequence of interest, which is heterolog in relation to said mesophyll- and/or epidermis-preferential or mesophyll- and/or epidermis-specific transcription regulating nucleotide sequence.
Dependencies are inferred from the claim text.