Method of screening antiobesity agents and animal model of obesity
OwnerKunio Yasunagainventor
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Abstract
A promoter for an angiopoietin-related growth factor (AGF), a vector comprising the promoter, a transformant comprising the promoter, and a method for screening an antiobesity agent, an antidiabetic agent, and/or a hypolipidemic agent by using the transformant, are disclosed. Further, an AGF knockout animal useful as an animal model for obesity, diabetes, and/or hyperlipemia is disclosed. Furthermore, an AGF transgenic animal useful for identification of a target molecule in a new drug development and/or a therapeutic agent for obesity, diabetes, and/or hyperlipemia is disclosed.
Claims8
(a) A DNA exhibiting a promoter activity for an angiopoietin-related growth factor, and consisting of a nucleotide sequence in which 1 to 10 nucleotides are substituted, deleted, added, and/or inserted in the nucleotide sequence consisting of nucleotides 2705-3001 of SEQ ID NO:
- 1, or
- (b) a DNA exhibiting a promoter activity for an angiopoietin-related growth factor, and consisting of a nucleotide sequence having a 90% or more homology with that consisting of nucleotides 2705-3001 of SEQ ID NO:
- 1.
A DNA consisting of the nucleotide sequence consisting of nucleotides 2705-3001 of SEQ ID NO:
- 1.
A nonhuman knockout animal characterized in that a polynucleotide encoding an angiopoietin-related growth factor is functionally deficient on a chromosome.
A nonhuman transgenic animal which is a nonhuman animal or an offspring animal thereof obtained by ontogenesis from totipotent cells in which a polynucleotide is introduced together with a CAG promoter, wherein the polynucleotide is carried on a chromosome, a polypeptide encoded by the polynucleotide is expressed in a somatic cell, and the polypeptide is selected from the group consisting of:
- (a) a polypeptide exhibiting an activity of suppressing an increase in body weight, and comprising an amino acid sequence consisting of amino acids 1-450 of SEQ ID NO:
- 3 or amino acids 1-433 of SEQ ID NO:
- 5,
- (b) a polypeptide exhibiting an activity of suppressing an increase in body weight, and comprising an amino acid sequence in which 1 to 10 amino acids are substituted, deleted, and/or inserted in an amino acid sequence consisting of amino acids 1-450 of SEQ ID NO:
- 3 or amino acids 1-433 of SEQ ID NO:
- 5,
- (c) a polypeptide exhibiting an activity of suppressing an increase in body weight, and encoded by a DNA which hybridizes under stringent conditions to a DNA encoding an amino acid sequence consisting of amino acids 1-450 of SEQ ID NO:
- 3 or amino acids 1-433 of SEQ ID NO:
- 5, and
- (d) a polypeptide exhibiting an activity of suppressing an increase in body weight, and comprising an amino acid sequence having a 95% or more homology with that consisting of amino acids 1-450 of SEQ ID NO:
- 3 or amino acids 1-433 of SEQ ID NO:
- 5.
Dependencies are inferred from the claim text.