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US 2006/0154340 A1Published application
Expression of potato proteinase inhibitor II in microbial hosts
OwnerMichael Louie, Peter Salamoneinventors
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Abstract
Multiple microbial host genetic systems with various molecular constructs enabling subcellular targeting, fusion protein generation and co-expression of ancillary enzymatic activities are used in the expression of potato proteinase inhibitor II. The expression levels achieved exceed previous reports by up to 10 to 1000 fold.
Claims4
A method of producing potato proteinase inhibitor II, comprising the steps of:
- (a) culturing a microbial host, wherein said microbial host comprises an expression unit comprising a DNA segment encoding potato proteinase inhibitor II heterologous to the microbial host, under conditions in which said potato proteinase inhibitor II is expressed into the culture medium;
- and
- (b) recovering not less than 500 mg of said potato proteinase inhibitor II per liter of culture medium.
Dependencies are inferred from the claim text.